PXD031845

PXD031845 is an original dataset announced via ProteomeXchange.

Title	Cell Surface Engineering Enables Surfaceome Profiling
Description	Understanding which proteins are presented at the cell surface is essential for ongoing therapeutic development, and to expand our basic understanding of biology. We envisioned the integration of cell surface engineering with radical-mediated protein biotinylation to profile cell surface proteins (CSPs). This method relies on the pre-functionalization of cells with cholesterol lipid groups, followed by their conjugation with an APEX2 ascorbate peroxidase enzyme. The APEX2 enzyme allows for the covalent conjugation of biotin moieties to nearby residues for subsequent enrichment, and serves as a tool for the enrichment and subsequent MS analysis of CSPs.
HostingRepository	PRIDE
AnnounceDate	2022-05-06
AnnouncementXML	Submission_2022-05-06_09:16:44.196.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zak Vilen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-23 05:36:37	ID requested
⏵ 1	2022-05-06 09:16:44	announced

Vilen Z, Reeves AE, O'Leary TR, Joeh E, Kamasawa N, Huang ML, Cell Surface Engineering Enables Surfaceome Profiling. ACS Chem Biol, 18(4):701-710(2023) [pubmed]

submitter keyword: Surfaceome, PDAC, Proximity Labeling

Mia Lace Huang
contact affiliation	Molecular Medicine, Huang Lab, Scripps Research, United States
contact email	miahuang@scripps.edu
lab head
Zak Vilen
contact affiliation	Scripps Research
contact email	Zvilen@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031845

PRIDE project URI

• PRIDE
  1. PXD031845
     1. Label: PRIDE project
     2. Name: Cell Surface Engineering Enables Surfaceome Profiling