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PXD031842

PXD031842 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity
DescriptionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causal pathogen of the ongoing global pandemic of coronavirus disease 2019 (COVID-19). Loss of smell and taste are new identified symptoms of COVID‐19, and both of these are related to cilia dysfunction. However, the pathological mechanisms are still unknown. Here, we found that SARS-CoV-2 ORF10 increases the overall E3 ligase activity of the CUL2ZYG11B complex by acting as a binding nexus for both ZYG11B and UBE2D1. Enhanced CUL2ZYG11B activity by ORF10 causes increased ubiquitination and subsequent proteasome-mediated degradation of an intraflagellar transport (IFT) complex B protein, IFT46, thereby impairing the cilia biogenesis and maintenance. Furthermore, we show that exposure of the respiratory tract of hACE2 humanized mice to SARS-CoV-2 ORF10 (using lentivirus pseuodotyped particles) results in multiple pathogenic phenotypes, including an obviously damaged cilia layer in the trachea, interstitial congestion, epithelial damage, and per
HostingRepositoryiProX
AnnounceDate2022-02-23
AnnouncementXMLSubmission_2022-10-08_00:36:54.221.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHaotian Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-23 04:23:31ID requested
12022-06-05 05:36:18announced
22022-10-08 00:36:56announced2022-10-08: Update publication information.
Publication List
Wang L, Liu C, Yang B, Zhang H, Jiao J, Zhang R, Liu S, Xiao S, Chen Y, Liu B, Ma Y, Duan X, Guo Y, Guo M, Wu B, Wang X, Huang X, Yang H, Gui Y, Fang M, Zhang L, Duo S, Guo X, Li W, SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity. J Cell Biol, 221(7):(2022) [pubmed]
Keyword List
submitter keyword: SARS-CoV-2 ORF10,cilia,LC-MS/MS
Contact List
Xuejiang Guo
contact affiliationState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University
contact emailguo_xuejiang@njmu.edu.cn
lab head
Haotian Zhang
contact affiliationState Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University
contact emailzhtclearlove@163.com
dataset submitter
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iProX dataset URI