PXD031842

PXD031842 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity
Description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causal pathogen of the ongoing global pandemic of coronavirus disease 2019 (COVID-19). Loss of smell and taste are new identified symptoms of COVID‐19, and both of these are related to cilia dysfunction. However, the pathological mechanisms are still unknown. Here, we found that SARS-CoV-2 ORF10 increases the overall E3 ligase activity of the CUL2ZYG11B complex by acting as a binding nexus for both ZYG11B and UBE2D1. Enhanced CUL2ZYG11B activity by ORF10 causes increased ubiquitination and subsequent proteasome-mediated degradation of an intraflagellar transport (IFT) complex B protein, IFT46, thereby impairing the cilia biogenesis and maintenance. Furthermore, we show that exposure of the respiratory tract of hACE2 humanized mice to SARS-CoV-2 ORF10 (using lentivirus pseuodotyped particles) results in multiple pathogenic phenotypes, including an obviously damaged cilia layer in the trachea, interstitial congestion, epithelial damage, and per
HostingRepository	iProX
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-10-08_00:36:54.221.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haotian Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-23 04:23:31	ID requested
1	2022-06-05 05:36:18	announced
⏵ 2	2022-10-08 00:36:56	announced	2022-10-08: Update publication information.

Wang L, Liu C, Yang B, Zhang H, Jiao J, Zhang R, Liu S, Xiao S, Chen Y, Liu B, Ma Y, Duan X, Guo Y, Guo M, Wu B, Wang X, Huang X, Yang H, Gui Y, Fang M, Zhang L, Duo S, Guo X, Li W, SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity. J Cell Biol, 221(7):(2022) [pubmed]

submitter keyword: SARS-CoV-2 ORF10,cilia,LC-MS/MS

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University
contact email	guo_xuejiang@njmu.edu.cn
lab head
Haotian Zhang
contact affiliation	State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University
contact email	zhtclearlove@163.com
dataset submitter

iProX dataset URI