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PXD031829 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometry-based analysis of kindlin phosphorylation, ubiquitination and interactome
DescriptionKindlin is an essential activator of integrins and thus indispensable in cell to extracellular matrix (ECM) adhesion. Kindlin abundance and functions are tightly regulated by multiple mechanisms including post-translational modifications (PTMs). Despite the critical roles of kindlin during embryonic development and pathogenesis, the knowledge of kindlin’s PTMs and interactome is far from complete. Here we analyze the phosphorylation, ubiquitination and interacting partners of kindlin in flat, firmly adherent interphase cells and round, weakly attached mitotic cells by immunoprecipitation-based mass spectrometry (IP-MS). The analysis generated potential phosphorylation and ubiquitination sites as well as interactors of kindlin and paved the way to investigate PTMs and binding partners of kindlin.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNanpeng Chen
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-22 08:49:30ID requested
12022-02-22 12:23:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mass spectrometry, kindlin, phosphorylation, ubiquitination
Contact List
Reinhard Fässler
contact affiliationMax Planck Institute for Biochemistry
contact emailfaessler@biochem.mpg.de
lab head
Nanpeng Chen
contact affiliationMax Planck Institute for Biochemistry
contact emailnchen@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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