PXD031828

PXD031828 is an original dataset announced via ProteomeXchange.

Title	Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization
Description	An advantageous alternative to the use of detergents in biochemical studies on membrane proteins have recently been styrene-maleic acid (SMA) amphipathic copolymers. These cut the membrane into nanodiscs (often called SMA-lipid particles, SMALPs), which contain membrane proteins surrounded by their relatively native lipid environment. In our recent study [1] we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:58:02.876.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031828
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-22 08:49:10	ID requested
1	2023-07-26 04:14:38	announced
2	2023-11-14 09:04:28	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:58:03	announced	2024-10-22: Updated project metadata.

Harant K, Č, ajka T, Angelisov, á P, Pokorn, á J, Ho, ř, ej, š, í V, Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization. Biophys Chem, 296():106989(2023) [pubmed]

10.1016/j.bpc.2023.106989;

10.6019/PXD031828;

submitter keyword: SMA, lipid rafts

Václav Hořejší
contact affiliation	Institute of Molecular Genetics of the Czech Academy of Sciences, Vídeňská 1083 142 20 Praha 4, Czechia
contact email	vaclav.horejsi@img.cas.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD031828

PRIDE project URI

• PRIDE
  1. PXD031828
     1. Label: PRIDE project
     2. Name: Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization