PXD031826

PXD031826 is an original dataset announced via ProteomeXchange.

Title	A method to quantitatively measure the proteostasis capacity of cells
Description	Protein homeostasis, or proteostasis, refers to the maintenance of the conformational and functional integrity of the proteome. The term proteostasis therefore encompasses all of the pathways that regulate the synthesis, concentration, folding, trafficking and degradation of proteins. The proteostasis capacity of a cell can be defined as the ability to prevent protein accumulation and aggregation into intracellular inclusions. Thus, proteostasis capacity is highly dependent on the protein quality control network. The ability to quantitatively measure the proteostasis capacity of a cell is an important step towards deciphering why some cell types are more susceptible to the formation of inclusions than others. This work demonstrates that FlucDM forms inclusions more readily in NSC-34 cells compared to Neuro-2a cells. The inclusions formed by FlucDM 94 in both Neuro-2a and NSC-34 cells were purified in order to identify differences in the types of proteins that engage with aggregation prone proteins in these cells. Overall, this work represents a first step towardsusing a model aggregation-prone protein to quantitatively measure the proteostasis capacity of cells, and thus understanding why some cells are vulnerable to proteome dysfunction, leading to the formation of inclusions.
HostingRepository	PRIDE
AnnounceDate	2026-02-19
AnnouncementXML	Submission_2026-02-19_03:41:04.918.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031826
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Albert Lee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-22 08:47:43	ID requested
⏵ 1	2026-02-19 03:41:05	announced

10.1016/J.BBAMCR.2026.120124;

10.6019/PXD031826;

submitter keyword: Protein aggregation, Inclusions, Proteostasis capacity, Neurodegenerative disorders, Aggregation index

Albert Lee
contact affiliation	Centre for Motor Neuron Disease Research, Macquarie Medical School, Macquarie University, North Ryde, NSW 2109 Australia
contact email	albert.lee@mq.edu.au
lab head
Albert Lee
contact affiliation	Macquarie University
contact email	albert.lee@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031826
     1. Label: PRIDE project
     2. Name: A method to quantitatively measure the proteostasis capacity of cells