PXD031825 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Co-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R |
Description | Co-IP of HIV-1 integrase proteins - WT, K258R and K258/264/266/273R. Integrase proteins were cloned into a mammalian expression vector (pJET). All proteins had an N-terminal HA-tag used for IP. As a negative control, cells were transfected with an empty HA vector. Co-IPs were done in HEK293T cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-20 |
AnnouncementXML | Submission_2022-05-20_07:07:32.168.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shelby Winans |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-22 08:47:18 | ID requested | |
⏵ 1 | 2022-05-20 07:07:32 | announced | |
Publication List
Winans S, Yu HJ, de Los Santos K, Wang GZ, KewalRamani VN, Goff SP, A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun, 13(1):1474(2022) [pubmed] |
Keyword List
submitter keyword: HIV-1, K258R |
Contact List
Stephen P. Goff |
contact affiliation | Department of Biochemistry and Molecular Biophysics Columbia University Medical Center, New York, NY Department of Microiology and Immunology Columbia University Medical Center, New York, NY |
contact email | spg1@cumc.columbia.edu |
lab head | |
Shelby Winans |
contact affiliation | Columbia University Medical Center |
contact email | zbretton@utexas.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031825 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031825
- Label: PRIDE project
- Name: Co-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R