PXD031825

PXD031825 is an original dataset announced via ProteomeXchange.

Title	Co-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R
Description	Co-IP of HIV-1 integrase proteins - WT, K258R and K258/264/266/273R. Integrase proteins were cloned into a mammalian expression vector (pJET). All proteins had an N-terminal HA-tag used for IP. As a negative control, cells were transfected with an empty HA vector. Co-IPs were done in HEK293T cells.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_07:07:32.168.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shelby Winans
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-22 08:47:18	ID requested
⏵ 1	2022-05-20 07:07:32	announced

Winans S, Yu HJ, de Los Santos K, Wang GZ, KewalRamani VN, Goff SP, A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun, 13(1):1474(2022) [pubmed]

submitter keyword: HIV-1, K258R

Stephen P. Goff
contact affiliation	Department of Biochemistry and Molecular Biophysics Columbia University Medical Center, New York, NY Department of Microiology and Immunology Columbia University Medical Center, New York, NY
contact email	spg1@cumc.columbia.edu
lab head
Shelby Winans
contact affiliation	Columbia University Medical Center
contact email	zbretton@utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031825

PRIDE project URI

• PRIDE
  1. PXD031825
     1. Label: PRIDE project
     2. Name: Co-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R