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PXD031825

PXD031825 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCo-immunoprecipitation of proteins with WT HIV-1 IN, K258R HIV-IN, and K258/264/266/273R
DescriptionCo-IP of HIV-1 integrase proteins - WT, K258R and K258/264/266/273R. Integrase proteins were cloned into a mammalian expression vector (pJET). All proteins had an N-terminal HA-tag used for IP. As a negative control, cells were transfected with an empty HA vector. Co-IPs were done in HEK293T cells.
HostingRepositoryPRIDE
AnnounceDate2022-05-20
AnnouncementXMLSubmission_2022-05-20_07:07:32.168.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShelby Winans
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-22 08:47:18ID requested
12022-05-20 07:07:32announced
Publication List
Winans S, Yu HJ, de Los Santos K, Wang GZ, KewalRamani VN, Goff SP, A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats. Nat Commun, 13(1):1474(2022) [pubmed]
Keyword List
submitter keyword: HIV-1, K258R
Contact List
Stephen P. Goff
contact affiliationDepartment of Biochemistry and Molecular Biophysics Columbia University Medical Center, New York, NY Department of Microiology and Immunology Columbia University Medical Center, New York, NY
contact emailspg1@cumc.columbia.edu
lab head
Shelby Winans
contact affiliationColumbia University Medical Center
contact emailzbretton@utexas.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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