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PXD031822

PXD031822 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTRRAP-interacting proteins during heat shock
DescriptionProject description: HeLa cells and cells stably expressing hTRRAP-3xFlAG were subjected to mass spectrometry (MS) analysis. Briefly, Cells were treated or untreated with heat shock at 42°C for 30 min, and TRRAP was immunoprecipitated from these cell extracts with 60 ul anti-FLAG M2 affinity gels by rotating at 4°C for 3 h. Immunoprecipitated sample was eluted by 40 ul FLAG peptide at 4°C for 30 min. Protein samples were loaded on 12% SDS-PAGE for preparation of gel slices. Gel bands were cut-out and subjected to in-gel digestion with trypsin. Resulting peptides were dissolved in a solution containing 0.1% trifluoroacetic acid and 2% acetonitrile and analyzed by an LTQ Orbitrap Velos Pro mass spectrometer coupled with a nanoLC instrument and HTC-PAL autosampler.
HostingRepositoryjPOST
AnnounceDate2022-06-19
AnnouncementXMLSubmission_2022-09-18_02:55:22.264.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMitsuaki Fujimoto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-21 23:03:58ID requested
12022-06-19 05:12:44announced
22022-09-18 02:55:22announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TRRAP, heat shock
Contact List
Nakai Akira
lab head
Mitsuaki Fujimoto
contact affiliationDepartment of Biochemistry and Molecular Biology, Graduate School of Medicine, Yamaguchi University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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