PXD031821
PXD031821 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HSF1-interacting proteins in control and heat shock conditions |
| Description | HeLa cells were treated or untreated with heat shock at 42°C for 5, 30, and 60 min, and were then fixed in medium containing 1% formaldehyde at 37°C for 30 min. Chromatin fraction was sonicated with the Branson Sonifier 450 (BRANSON Ultrasonics) into fragmented DNA around 200-300 bp. HSF1 bound to genomic DNA was immunoprecipitated with 2 ul of rabbit immune serum for mHSF1 (anti-mHSF1j or Millipore ABE1044) or preimmune serum conjugated to Dynabeads Protein A at 4°C for 3 h (ChIP). Immunoprecipitated sample was eluted by incubation at 90°C for 30 min in 1× Laemmli sample buffer (2% SDS, 60 mM Tris-HCl (pH 6.8), 100 mM DTT, 10% glycerol, 0.001% bromophenol blue). Protein samples were loaded on 12% SDS-PAGE for preparation of gel slices for mass spectrometry (MS). Gel bands were cut-out and subjected to in-gel digestion with trypsin. Resulting peptides were dissolved in a solution containing 0.1% trifluoroacetic acid and 2% acetonitrile and analyzed by an LTQ Orbitrap Velos Pro mass spectrometer coupled with a nanoLC instrument and HTC-PAL autosampler. |
| HostingRepository | jPOST |
| AnnounceDate | 2022-06-19 |
| AnnouncementXML | Submission_2022-09-18_03:15:21.065.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mitsuaki Fujimoto |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-02-21 22:40:27 | ID requested | |
| 1 | 2022-06-19 05:12:12 | announced | |
| ⏵ 2 | 2022-09-18 03:15:21 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: HSF1, CHIP-MS, heat shock |
Contact List
| Nakai Akira | |
|---|---|
| lab head | |
| Mitsuaki Fujimoto | |
| contact affiliation | Department of Biochemistry and Molecular Biology, Graduate School of Medicine, Yamaguchi University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001496/ |
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