PXD031819

PXD031819 is an original dataset announced via ProteomeXchange.

Title	Dual-Resolving of Positional and Geometric Isomerism of C=Cs via Bifunctional Visible-light-activated Photocycloaddition-Photoisomerization Reaction System
Description	The biological functions of lipids largely depend on their chemicalspatial structures. The position and configuration of C=C bonds are two of the essential attributes that determine the structures of unsaturated lipids. However, simultaneous identification of both attributes remains challenging. Here, we developed a bifunctional visible-light-activated photocycloaddition-photoisomerization reaction system, which enabled the dual-resolving of the positional and geometric isomerism of C=C bonds in lipids when combined with liquid chromatographic mass spectrometry. The dual-pathway reaction mechanism was demonstrated by experiments and density functional theory calculations. Based on this bifunctional reaction system, a workflow of deep structural lipidomics was established, and allowed the revealing of unique patterns of cis-trans-isomers in bacteria, as well as the tracking of C=C positional isomers changes in mouse brain ischemia. This study not only offers a powerful tool for deep lipid structural biology, but also provides a new paradigm for developing the multifunctional visible-light-induced reaction.
HostingRepository	iProX
AnnounceDate	2022-02-19
AnnouncementXML	Submission_2022-10-08_00:33:22.405.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guifang Feng
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 19:09:11	ID requested
1	2022-02-21 19:09:49	announced
⏵ 2	2022-10-08 00:33:23	announced	2022-10-08: Update publication information.

Feng G, Gao M, Wang L, Chen J, Hou M, Wan Q, Lin Y, Xu G, Qi X, Chen S, Dual-resolving of positional and geometric isomers of C=C bonds via bifunctional photocycloaddition-photoisomerization reaction system. Nat Commun, 13(1):2652(2022) [pubmed]

submitter keyword: lipidomic, lisomers, Photocycloaddition, Photoisomerization

Suming Chen
contact affiliation	The Institute for Advanced Studies, Wuhan University
contact email	chensm@iccas.ac.cn
lab head
Guifang Feng
contact affiliation	The Institute for Advanced Studies, Wuhan University
contact email	gffeng@whu.edu.cn
dataset submitter

iProX dataset URI