PXD031816

PXD031816 is an original dataset announced via ProteomeXchange.

Title	Conoid extrusion serves as gatekeeper for entry of glideosome components into the pellicular space to control motility and invasion in Apicomplexa
Description	Members of the apicomplexans are defined by apical cytoskeletal structures and secretory or-ganelles, tailored for motility and invasion. Gliding is powered by the actomyosin-dependent rearward translocation of apically secreted transmembrane adhesins. In Toxoplasma gondii, the conoid, composed of a cone of spiraling tubulin fibers and apposed preconoidal rings (PCRs), is an enigmatic, dynamic organelle of undefined function. Here we mapped five new components of the PCRs and deduce that the structure serves as a pivotal hub for actin polymerization and glideosome assembly. F-actin produced by Formin1 on the PCRs is used by Myosin H to generate the force for conoid extrusion. A set of conserved B-box-type zinc finger domain containing proteins in Apicomplexa is indispensable for PCRs formation, co-noid extrusion and motility in Toxoplasma and Plasmodium. Conoid dynamics directs the flux of F-actin to the pellicular space, acting as dynamic gatekeeper to tightly control parasite motility during invasion and egress.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:00.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oscar Vadas
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 14:28:49	ID requested
1	2023-08-31 08:40:43	announced
⏵ 2	2023-11-14 09:05:04	announced	2023-11-14: Updated project metadata.

Dos Santos Pacheco N, Brusini L, Haase R, Tosetti N, Maco B, Brochet M, Vadas O, Soldati-Favre D, Conoid extrusion regulates glideosome assembly to control motility and invasion in Apicomplexa. Nat Microbiol, 7(11):1777-1790(2022) [pubmed]

submitter keyword: Apicomplexa, conoid extrusion, egress, motility, AlphaFold, F-actin, invasion, Toxoplasma gondii, HDX-MS, Plasmodium berghei, preconoidal rings, micronemes

Oscar Vadas
contact affiliation	University of Geneva, Department of Molecular Microbiology, Geneva, Switzerland
contact email	oscar.vadas@unige.ch
lab head
Oscar Vadas
contact affiliation	University of Geneva, Faculty of Medicine
contact email	oscar.vadas@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD031816

PRIDE project URI

• PRIDE
  1. PXD031816
     1. Label: PRIDE project
     2. Name: Conoid extrusion serves as gatekeeper for entry of glideosome components into the pellicular space to control motility and invasion in Apicomplexa