PXD031803 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Reduction of Phosphoribulokinase Amount and Re-Routing Metabolism in Chlamydomonas reinhardtii CP12 Mutants |
Description | The chloroplast protein CP12 is involved in the dark/light regulation of the Calvin-Benson-Bassham cycle, and in particular in the dark inhibition of two enzymes: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK), but other functions related to stress have been proposed. We knocked out the unique CP12 gene to prevent its expression in Chlamydomonas reinhardtii (ΔCP12). The growth rates of both wild-type and ΔCP12 cells were nearly identical, as was the GAPDH protein abundance and activity in both cell lines. On the contrary, the abundance of PRK and its specific activity were significantly reduced in ΔCP12, as revealed by relative quantitative proteomics. Isolated PRK lost irreversibly its activity over-time in vitro, that was prevented in the presence of recombinant CP12 in a redox-independent manner. We have identified amino acid residues in the CP12 protein that are required for this new function preserving PRK activity. Numerous proteins involved in redox homeostasis and stress responses were more abundant and the expressions of various metabolic pathways were also increased or decreased in the absence of CP12. These results highlight CP12 as a moonlighting protein with additional functions beyond its well-known regulatory role in carbon metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-27 |
AnnouncementXML | Submission_2022-05-27_02:49:40.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Regine Lebrun |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-21 05:39:52 | ID requested | |
⏵ 1 | 2022-05-27 02:49:41 | announced | |
Publication List
G, é, rard C, Lebrun R, Lemesle E, Avilan L, Chang KS, Jin E, Carri, è, re F, Gontero B, Launay H, CP12 Mutants. Int J Mol Sci, 23(5):(2022) [pubmed] |
Keyword List
submitter keyword: Calvin Benson Bassham, CP12, enzymatic activity, intrinsically disordered protein, quantitative proteomics, photosynthesis, glyoxylate pathway, carbon allocation, nitrogen uptake |
Contact List
Lebrun Regine |
contact affiliation | Proteomic Platform of Institut de Microbiologie de la Méditerranée CNRS 3479 |
contact email | rlebrun@imm.cnrs.fr |
lab head | |
Regine Lebrun |
contact affiliation | CNRS |
contact email | rlebrun@imm.cnrs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031803
- Label: PRIDE project
- Name: Reduction of Phosphoribulokinase Amount and Re-Routing Metabolism in Chlamydomonas reinhardtii CP12 Mutants