PXD031800

PXD031800 is an original dataset announced via ProteomeXchange.

Title	Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.
Description	Altered thermal solubility measurement techniques are emerging as powerful tools to assess ligand binding, post-translational modification, protein-protein interactions, and many other cellular processes that affect protein state under various cellular conditions. Thermal solubility or stability profiling techniques are enabled on a global proteomic scale by employing the latest generation of isobaric tagging reagents that facilitate multiplexing capacity required to measure the proteome across thermal gradients. Key among these is thermal proteomic profiling (TPP), which requires 8-10 isobaric tags per gradient and multiple sets of proteomic analyses to measure different replicates and conditions. Furthermore, using thermal profiling techniques to measure protein state across different conditions also requires measurements of difference in protein abundance. Here, we use PISA to assess protein abundance and thermal stability.
HostingRepository	PRIDE
AnnounceDate	2024-02-14
AnnouncementXML	Submission_2024-02-14_09:03:05.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	J Patrick Murphy
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 05:36:01	ID requested
⏵ 1	2024-02-14 09:03:06	announced

10.1002/pmic.202100254;

Sultonova M, Blackmore B, Du R, Philips O, Paulo JA, Murphy JP, Integrated changes in thermal stability and proteome abundance during altered nutrient states in Escherichia coli and human cells. Proteomics, 22(19-20):e2100254(2022) [pubmed]

submitter keyword: PISA, thermal proteomic profiling

J Patrick Murphy
contact affiliation	Department of Biology University of Prince Edward Island
contact email	jpmurphy@upei.ca
lab head
J Patrick Murphy
contact affiliation	University of Prince Edward Island
contact email	jpmurphy@upei.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD031800

PRIDE project URI

• PRIDE
  1. PXD031800
     1. Label: PRIDE project
     2. Name: Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.Integrated changes in thermal stability and proteome abundance during altered growth and metabolic cell states.