PXD031795

PXD031795 is an original dataset announced via ProteomeXchange.

Title	Chemerin effect on the endometrial proteome of the domestic pig during implantation obtained by LC-MS/MS analysis
Description	Chemerin (CHEM) is a hormone mainly expressed in adipocytes involved in the regulation of energy homeostasis and inflammatory response. CHEM expression has been demonstrated in the structures of the porcine hypothalamic-pituitary-gonadal axis, as well as in the uterus, trophoblasts and conceptuses of pigs. In this study, we performed high-throughput proteomic analyses (Liquid Chromatography with Tandem Mass Spectrometry, LC-MS/MS) to examine the influence of CHEM (400 ng/ml) on differentially regulated proteins (DRPs) in the porcine endometrial tissue explants during implantation (15 to 16 days of gestation). Among all 352 DRPs, 164 were up-regulated and 188 were down-regulated in CHEM-treated group. DRPs were assigned to 47 gene ontology (GO) terms (p-adjusted < 0.05). Validation of four DRPs (IFIT5, TGFβ1, ACO1 and PGRMC1) by Western blot analysis confirmed the veracity and accuracy of LC-MS/MS method used in the present study. We suggest that CHEM, by modulating various protein expressions takes part in the endometrial cell proliferation, migration and invasion at the time of implantation. It also regulates the endometrial immune response, sensitivity to P4 and formation of new blood vessels. Additionally, CHEM appears to be an important factor involved in endothelial cell dysfunction during the pathogenesis of preeclampsia. The identification of a large number of DRPs under the influence of CHEM provides a valuable resource for understanding the molecular mechanisms of this hormone during implantation, which is a prerequisite for better control of pig reproduction.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:39.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 05:30:25	ID requested
1	2022-04-26 16:49:29	announced
⏵ 2	2023-11-14 08:55:40	announced	2023-11-14: Updated project metadata.

Orzechowska K, Dobrzy, ń K, Kie, ż, un M, Malinowska A, Ś, widerska B, Kami, ń, ski T, Smoli, ń, ska N, Chemerin Effect on the Endometrial Proteome of the Domestic Pig during Implantation Obtained by LC-MS/MS Analysis. Cells, 11(7):(2022) [pubmed]

submitter keyword: endometrium, TMT, LC-MS, chemerin,pig

Nina Smolińska
contact affiliation	Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury
contact email	nina.smolinska@uwm.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031795
     1. Label: PRIDE project
     2. Name: Chemerin effect on the endometrial proteome of the domestic pig during implantation obtained by LC-MS/MS analysis