PXD031787

PXD031787 is an original dataset announced via ProteomeXchange.

Title	Differential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E
Description	Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants. To start filling these gaps, we devised a differential centrifugation set up, where we enriched intact autophagosomes and performed affinity purification-mass spectrometry (AP-MS), using ATG8 as a bait. This method enabled the identification of an autophagy adaptor protein.
HostingRepository	PRIDE
AnnounceDate	2022-10-18
AnnouncementXML	Submission_2022-10-18_08:22:40.998.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RichardImre
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	methylthiolated residue; ubiquitination signature dipeptidyl lysine; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 03:31:59	ID requested
⏵ 1	2022-10-18 08:22:41	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, LC-MSMS,Autophagosomes, affinity purification

YasinDagdas
contact affiliation	Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna BioCenter (VBC), Vienna, Austria.
contact email	yasin.dagdas@gmi.oeaw.ac.at
lab head
RichardImre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031787

PRIDE project URI

• PRIDE
  1. PXD031787
     1. Label: PRIDE project
     2. Name: Differential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E