PXD031772

PXD031772 is an original dataset announced via ProteomeXchange.

Title	Enrichment of NICD4 interacting proteins by three different methods.
Description	There are several methods to bring down the bait and interactors from the cell lysates, each with its own advantages and disadvantages. Recently, the most commonly used method is one step AP, TAP and proximity-labeling. In order to analyze the Strengths and weaknesses of the three methods, fusion proteins were constructed by NICD4 with FLAG, SFB (S tag-2x Flag tag-SBP tag) and TurboID respectively, NICD4 and its interactors were obtained by purification. By analyzing these proteins, we believe that SFB-TAP is the most reliable and effective method to identify interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_00:00:11.506.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031772
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	bian weixiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-21 02:43:07	ID requested
⏵ 1	2022-08-12 00:00:12	announced

Bian W, Jiang H, Feng S, Chen J, Wang W, Li X, Protocol for establishing a protein-protein interaction network using tandem affinity purification followed by mass spectrometry in mammalian cells. STAR Protoc, 3(3):101569(2022) [pubmed]

submitter keyword: NICD4, SFB-TAP, FLAG tag AP, TurboID.

Xu Li
contact affiliation	School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China
contact email	lixu@westlake.edu.cn
lab head
bian weixiang
contact affiliation	Westlake University
contact email	bianweixiang@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031772

PRIDE project URI

• PRIDE
  1. PXD031772
     1. Label: PRIDE project
     2. Name: Enrichment of NICD4 interacting proteins by three different methods.