PXD031736

PXD031736 is an original dataset announced via ProteomeXchange.

Title	Food vacuole proteome of P. berghei heme pathway ferrochelatase knockout parasite by LC-MS/MS
Description	LC-MS/MS of in-solution trypsin digested FV protein extracts was carried out for the FVs isolated from PbWT and PbFCKO parasites. The FV preparations had signature FV proteins such as plasmepsin IV, berghepain, aminopeptidases, subunits of vacuolar-type H+ATPase (V-type H ATPase), together with parasitophorous vacuolar (PV) proteins including exported protein 1 (Exp1), Exp2, early transcribed membrane protein, PV1, PV5 (lipocalin) etc., and Rab GTPases associated with cytostome-FV trafficking. A total number of 251 and 201 proteins could be identified for WT (P1) and FCKO (P2) FVs, respectively, and 175 proteins were common between them suggesting an overall consistency in the preparations. The results obtained have suggested that the FV proteome in FCKO parasites is compromised. None of the subunits of V-type H+ATPase - a proton pump maintaining the acidic pH of FV could be detected in FCKO FVs indicating the lower abundance of these proteins. In addition, berghepain-2 - a cysteine protease involved in Hb degradation could not be detected in FCKO FVs.
HostingRepository	PRIDE
AnnounceDate	2022-05-10
AnnouncementXML	Submission_2022-05-09_22:11:37.238.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viswanathan Arun Nagaraj
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-17 04:08:51	ID requested
⏵ 1	2022-05-09 22:11:37	announced

Dataset with its publication pending

submitter keyword: Wildtype (WT), ferrochelatase (FC), knockout (KO), food vacuole (FV), Plasmodium berghei (Pb)

Arun Nagaraj
contact affiliation	Institute of Life Sciences, Bhubaneswar, India
contact email	arun@ils.res.in
lab head
Viswanathan Arun Nagaraj
contact affiliation	Institute of Life Sciences, Bhubaneswar
contact email	arun@ils.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031736
     1. Label: PRIDE project
     2. Name: Food vacuole proteome of P. berghei heme pathway ferrochelatase knockout parasite by LC-MS/MS