PXD031731

PXD031731 is an original dataset announced via ProteomeXchange.

Title	A Novel Differentiated HuH-7 Cell Model to Examine Bile Acid Metabolism, Transport and Cholestatic Hepatotoxicity
Description	Hepatic cell lines serve as economical and reproducible alternatives for primary human hepatocytes. However, the utility of hepatic cell lines to examine bile acid homeostasis and cholestatic toxicity is limited due to abnormal expression and function of bile acid-metabolizing enzymes, transporters, and the absence of canalicular formation. Previously, addition of dexamethasone (DEX) and Matrigel™ overlay restored expression, localization, and function of the bile salt export pump (BSEP), and formation of bile canalicular-like structures in four-week cultures of HuH-7 human hepatoma cells. We present here an improved differentiation process with the addition of 0.5% dimethyl sulfoxide (DMSO), which increased the expression and function of the major bile acid uptake and efflux transporters, sodium taurocholate co-transporting polypeptide (NTCP) and BSEP, respectively, in two-week HuH-7 cell cultures. This in vitro model was further characterized for expression of cytochrome P450 enzymes (CYP450s), uridine 5'-diphospho-glucuronosyltransferase (UGTs) and transporters using quantitative targeted proteomics.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:35:35.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chitra Saran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-17 03:45:18	ID requested
1	2022-10-14 12:25:51	announced
⏵ 2	2023-11-14 07:35:38	announced	2023-11-14: Updated project metadata.

Saran C, Fu D, Ho H, Klein A, Fallon JK, Honkakoski P, Brouwer KLR, A novel differentiated HuH-7 cell model to examine bile acid metabolism, transport and cholestatic hepatotoxicity. Sci Rep, 12(1):14333(2022) [pubmed]

submitter keyword: Human, Sciex, Quantitative Targeted Proteomics, HuH-7 Cells

Kim Brouwer
contact affiliation	Division of Pharmacotherapy and Experimental Therapeutics, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, USA
contact email	kbrouwer@unc.edu
lab head
Chitra Saran
contact affiliation	University of North Carolina at Chapel Hill
contact email	saranch1@email.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031731

PRIDE project URI

• PRIDE
  1. PXD031731
     1. Label: PRIDE project
     2. Name: A Novel Differentiated HuH-7 Cell Model to Examine Bile Acid Metabolism, Transport and Cholestatic Hepatotoxicity