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PXD031700

PXD031700 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleO-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling
DescriptionLigand-stimulated epidermal growth factor receptor (EGFR) signaling plays fundamental roles in normal cell physiology, such as cell growth, cell proliferation, and cell survival. Deregulation of EGFR signaling contributes to the development and progression of diseases including cancer. Despite its essential role in biology, the mechanisms by which EGFR signaling is regulated in cells are still poorly understood. Here, we demonstrate that O-linked N-acetyl-glucosamine (O-GlcNAc) modification serves as an important regulator of EGFR intracellular trafficking and degradation. Mechanistically, O-GlcNAcylation of hepatocyte growth factor regulated tyrosine kinase substrate (HGS), a key protein in EGFR intraluminal sorting pathway, inhibits HGS interaction with signal-transducing adaptor molecule (STAM), thereby impairing the formation of endosomal sorting complex required for transport-0 (ESCRT-0). Moreover, O-GlcNAcylation increases HGS ubiquitination and decreases its protein stability in cells. Consequently, HGS O-GlcNAcylation inhibits EGFR intraluminal sorting and lysosomal degradation, leading to the accumulation of EGFR and prolonged EGFR signaling in cells.
HostingRepositoryPRIDE
AnnounceDate2022-05-31
AnnouncementXMLSubmission_2022-05-31_06:19:31.728.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhiya Fan
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-16 01:54:18ID requested
12022-05-31 06:19:32announced
Publication List
Wu L, Cheng Y, Geng D, Fan Z, Lin B, Zhu Q, Li J, Qin W, Yi W, O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling. Proc Natl Acad Sci U S A, 119(10):e2107453119(2022) [pubmed]
Keyword List
submitter keyword: O-GlcNAcylation, EGFR, endosomal sorting, membrane receptors
Contact List
Weijije Qin
contact affiliationNation Center for Protein Sciences, Beijing
contact emailaunp_dna@126.com
lab head
Zhiya Fan
contact affiliationNational Center for Protein Sciences (Beijing)
contact emailzyfan7@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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