PXD031700 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling |
Description | Ligand-stimulated epidermal growth factor receptor (EGFR) signaling plays fundamental roles in normal cell physiology, such as cell growth, cell proliferation, and cell survival. Deregulation of EGFR signaling contributes to the development and progression of diseases including cancer. Despite its essential role in biology, the mechanisms by which EGFR signaling is regulated in cells are still poorly understood. Here, we demonstrate that O-linked N-acetyl-glucosamine (O-GlcNAc) modification serves as an important regulator of EGFR intracellular trafficking and degradation. Mechanistically, O-GlcNAcylation of hepatocyte growth factor regulated tyrosine kinase substrate (HGS), a key protein in EGFR intraluminal sorting pathway, inhibits HGS interaction with signal-transducing adaptor molecule (STAM), thereby impairing the formation of endosomal sorting complex required for transport-0 (ESCRT-0). Moreover, O-GlcNAcylation increases HGS ubiquitination and decreases its protein stability in cells. Consequently, HGS O-GlcNAcylation inhibits EGFR intraluminal sorting and lysosomal degradation, leading to the accumulation of EGFR and prolonged EGFR signaling in cells. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-31 |
AnnouncementXML | Submission_2022-05-31_06:19:31.728.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhiya Fan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-16 01:54:18 | ID requested | |
⏵ 1 | 2022-05-31 06:19:32 | announced | |
Publication List
Wu L, Cheng Y, Geng D, Fan Z, Lin B, Zhu Q, Li J, Qin W, Yi W, O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling. Proc Natl Acad Sci U S A, 119(10):e2107453119(2022) [pubmed] |
Keyword List
submitter keyword: O-GlcNAcylation, EGFR, endosomal sorting, membrane receptors |
Contact List
Weijije Qin |
contact affiliation | Nation Center for Protein Sciences, Beijing |
contact email | aunp_dna@126.com |
lab head | |
Zhiya Fan |
contact affiliation | National Center for Protein Sciences (Beijing) |
contact email | zyfan7@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031700
- Label: PRIDE project
- Name: O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling