PXD031700

PXD031700 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling
Description	Ligand-stimulated epidermal growth factor receptor (EGFR) signaling plays fundamental roles in normal cell physiology, such as cell growth, cell proliferation, and cell survival. Deregulation of EGFR signaling contributes to the development and progression of diseases including cancer. Despite its essential role in biology, the mechanisms by which EGFR signaling is regulated in cells are still poorly understood. Here, we demonstrate that O-linked N-acetyl-glucosamine (O-GlcNAc) modification serves as an important regulator of EGFR intracellular trafficking and degradation. Mechanistically, O-GlcNAcylation of hepatocyte growth factor regulated tyrosine kinase substrate (HGS), a key protein in EGFR intraluminal sorting pathway, inhibits HGS interaction with signal-transducing adaptor molecule (STAM), thereby impairing the formation of endosomal sorting complex required for transport-0 (ESCRT-0). Moreover, O-GlcNAcylation increases HGS ubiquitination and decreases its protein stability in cells. Consequently, HGS O-GlcNAcylation inhibits EGFR intraluminal sorting and lysosomal degradation, leading to the accumulation of EGFR and prolonged EGFR signaling in cells.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_06:19:31.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiya Fan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-16 01:54:18	ID requested
⏵ 1	2022-05-31 06:19:32	announced

Wu L, Cheng Y, Geng D, Fan Z, Lin B, Zhu Q, Li J, Qin W, Yi W, O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling. Proc Natl Acad Sci U S A, 119(10):e2107453119(2022) [pubmed]

submitter keyword: O-GlcNAcylation, EGFR, endosomal sorting, membrane receptors

Weijije Qin
contact affiliation	Nation Center for Protein Sciences, Beijing
contact email	aunp_dna@126.com
lab head
Zhiya Fan
contact affiliation	National Center for Protein Sciences (Beijing)
contact email	zyfan7@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD031700

PRIDE project URI

• PRIDE
  1. PXD031700
     1. Label: PRIDE project
     2. Name: O-GlcNAcylation regulates epidermal growth factor receptor intracellular trafficking and signaling