PXD031688

PXD031688 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SARS-CoV-2 Nsp1 N-terminal and linker regions as a platform for host translational shutoff_AP-MS_and_sucrose-gradient_datasets
Description	In the early stages of SARS-CoV-2 infection, non-structural protein 1 (Nsp1) inhibits the innate immune response by inserting its C-terminal helices into the mRNA entry channel of the ribosome and promoting mRNA degradation. However, the mechanism by which Nsp1 achieves host translational shutoff while allowing for viral protein synthesis remains unclear. As the conserved N-terminal domain (NTD) and linker region has been implicated with this process, we set out to characterize the interactome of Nsp1 and its topology by crosslinking mass spectrometry. The distance restraints allowed us to derive an integrative model of full-length Nsp1 on the 40S subunit. We find that Nsp1 is in contact with 40S proteins lining the mRNA entry channel and the G subunit of the eIF3 complex. We further show that Nsp1 predominantly binds to initiating ribosomes by affinity purification mass spectrometry and sucrose gradient ultracentrifugation. Our results point towards a potential mechanism by which Nsp1 is preferentially recruited to host-translating ribosomes, leading to selective inhibition of canonical initiation intermediates and subsequent mRNA degradation.
HostingRepository	jPOST
AnnounceDate	2023-02-15
AnnouncementXML	Submission_2023-02-14_07:00:14.019.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Schildhauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Q Exactive; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-15 02:45:55	ID requested
⏵ 1	2023-02-14 07:00:14	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: mass spectrometry, protein-protein interaction, AP-MS, SARS-CoV-2, NSP1, control, enrichment, sucrose gradient

submitter keyword: mass spectrometry, protein-protein interaction, AP-MS, SARS-CoV-2, NSP1, control, enrichment, sucrose gradient

Contact List

Juri Rappsilber
lab head
Fabian Schildhauer
contact affiliation	Technische Universität Berlin, Institute of Biotechnology, Bioanalytics, 13355 Berlin, Germany
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001478/