PXD031682

PXD031682 is an original dataset announced via ProteomeXchange.

Title	The Achromobacter xylosoxidans type VI secretion system 2 (T6SS): from clinical to molecular evidences of its 3 implication in cystic fibrosis
Description	Bacteria of the genus Achromobacter are environmental germs, with an unknown reservoir, which can become opportunistic pathogens in immunocompromised patients, and be responsible for bacteremia, meningitis, pneumonia and peritonitis. Achromobacter xylosoxidans is an emerging pathogenic bacterium frequently isolated in the context of cystic fibrosis (CF). Recent studies show that A. xylosoxidans is involved in the degradation of the respiratory function of CF patients. The respiratory ecosystem of CF patients is colonized by bacterial species that constantly fight for space and access to nutrients. In particular, these bacteria use an antagonism system, a type VI secretion nanomachine (T6SS), which represents a virulence factor in many pathogenic bacteria. This study aimed to investigate the prevalence of the T6SS genes in Achromobacter xylosoxidans isolated in cystic fibrosis patient. We also evaluated clinical and molecular characteristics of T6SS-positive A. xylosoxidans strains. We have shown that A. xylosoxidans possesses a T6SS-encoded gene cluster and that some environmental and clinical isolates assemble a functional T6SS nanomachine. The A. xylosoxidans T6SS is used to target competitor bacteria, including other CF-specific pathogens. We gathered some evidences pointing toward a role of T6SS in CF-lung colonization: i, CF mimicking conditions trigger the activation of A. xylosoxidans T6SS; ii, we detected Hcp in the sputum of CF patient and iii, the T6SS helps internalization of A. xylosoxidans in lung epithelial cells. Our study highlights a new clinical determinant of the virulence of A. xylosoxidans as well as new diagnostic and therapeutic options in cystic fibrosis.
HostingRepository	PRIDE
AnnounceDate	2024-02-01
AnnouncementXML	Submission_2024-02-01_03:37:54.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Regine Lebrun
SpeciesList	scientific name: Achromobacter xylosoxidans A8; NCBI TaxID: 762376;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-15 01:45:17	ID requested
⏵ 1	2024-02-01 03:37:54	announced

Dataset with its publication pending

submitter keyword: cystic fibrosis, proteomics, clinical microbiology,virulence factor, T6SS,Bacterial secretion system, bacterial competition

Régine Lebrun
contact affiliation	Aix Marseille Univ, CNRS, FR3479, Proteomics facility of IMM, Marseille Protéomique
contact email	rlebrun@imm.cnrs.fr
lab head
Regine Lebrun
contact affiliation	CNRS
contact email	rlebrun@imm.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031682
     1. Label: PRIDE project
     2. Name: The Achromobacter xylosoxidans type VI secretion system 2 (T6SS): from clinical to molecular evidences of its 3 implication in cystic fibrosis