PXD031664

PXD031664 is an original dataset announced via ProteomeXchange.

Title	LncRNA RUS shapes the gene expression program towards neurogenesis
Description	The evolution of brain complexity correlates with an increased expression of long, non-coding (lnc) RNAs in neuronal tissues. Although prominent examples illustrate the potential of lncRNAs to scaffold and target epigenetic regulators to chromatin loci, only few cases have been described to function during neurogenesis. We present a first functional characterization of the lncRNA LINC01322, which we term RUS for ‘RNA upstream of Slitrk3’. The RUS gene is well conserved in mammals by sequence and synteny next to the neurodevelopmental gene Slitrk3. RUS is exclusively expressed in neural cells and its expression increases along with neuronal markers during neuronal differentiation of mouse embryonic cortical neural stem cells. Depletion of RUS locks neuronal precursors in an intermediate state towards neuronal differentiation, with arrested cell cycle and increased apoptosis. RUS associates with chromatin in the vicinity of genes involved in neurogenesis, most of which change their expression upon RUS depletion. The identification of a range of epigenetic regulators as specific RUS interactors suggests that the lncRNA may mediate gene activation and repression in a highly context-dependent manner.
HostingRepository	PRIDE
AnnounceDate	2022-06-21
AnnouncementXML	Submission_2022-06-21_14:35:11.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-14 01:17:01	ID requested
⏵ 1	2022-06-21 14:35:12	announced

Schneider MF, M, ü, ller V, M, ü, ller SA, Lichtenthaler SF, Becker PB, Scheuermann JC, shapes the gene expression program towards neurogenesis. Life Sci Alliance, 5(10):(2022) [pubmed]

submitter keyword: RNA protein interaction

Stefan F. Lichtenthaler
contact affiliation	DZNE Munich Neuroproteomics Feodor-Lynen-Str. 17 81377 München Germany
contact email	stefna.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031664
     1. Label: PRIDE project
     2. Name: LncRNA RUS shapes the gene expression program towards neurogenesis