PXD031655
PXD031655 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitiave analysis of protein methylome reveals the cellular response to ionizing radiation |
| Description | Protein methylation plays important roles in DNA damage signaling. To date, there is still a lack of global profiling of whole-cell methylation changes during the DNA damage response and repair. In this study, using HILIC affinity enrichment combined with MS analysis, we conducted a quantitative analysis of the methylated proteins in HEK293T cells in response to IR-induced DNA damage. In total, 235 distinct methylation sites responding to IR treatment were identified, and 38% of them were previously unknown. Multiple RNA-binding proteins were differentially methylated upon DNA damage stress. Furthermore, we identified 14 novel methylations in DNA damage response-related proteins. Moreover, we validated the function of PARP1 K23 methylation in repairing IR-induced DNA lesions. |
| HostingRepository | iProX |
| AnnounceDate | 2022-02-14 |
| AnnouncementXML | Submission_2023-12-24_20:37:55.942.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chenxi Jia |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-02-13 21:11:24 | ID requested | |
| 1 | 2022-06-21 23:50:36 | announced | |
| ⏵ 2 | 2023-12-24 20:37:56 | announced | 2023-12-25: Update information. |
Publication List
| Wang X, Mi S, Zhao M, Lu C, Jia C, Chen Y, Quantitative Analysis of the Protein Methylome Reveals PARP1 Methylation is involved in DNA Damage Response. Front Mol Biosci, 9():878646(2022) [pubmed] |
Keyword List
| submitter keyword: Protein methylation, DNA damage response, HILIC-affinity enrichment |
Contact List
| Yali Chen | |
|---|---|
| contact affiliation | State Key Laboratory of Proteomics, National Center for Protein Sciences – Beijing, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing 102206, China |
| contact email | cyali_2000@163.com |
| lab head | |
| Chenxi Jia | |
| contact affiliation | Beijing Proteome Research Center/ National Center for Protein Sciences(Beijing) |
| contact email | cjia99@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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