PXD031649

PXD031649 is an original dataset announced via ProteomeXchange.

Title	Identification of interaction partners of the Toxoplasma gondii Cysteine Repeat Modular Proteins TGGT1_292020 and TGGT1_261080
Description	Apicomplexa are obligate intracellular parasites. While most species are restricted to specific hosts and cell types, Toxoplasma gondii can invade every nucleated cell derived from warm-blooded animals. This broad host range suggests that this parasite can recognize multiple host cell ligands or structures, leading to the activation of a central protein complex, which should be conserved in all apicomplexans. Cysteine Repeat Modular Proteins (CRMPs) are a family of apicomplexan specific proteins. In Toxoplasma gondii, two CRMPs are present in the genome. We performed pulldown of 3xHA tagged CRMPA and CRMPB. In a second dataset we performed biotinylation of TurboID tagged CRMPB on extracellular parasites to identify transient interaction partners. In a third dataset, we performed biotinylation of TurboID tagged CRMPA or B during invasion or in an invasion blocking buffer.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:44.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-13 13:42:51	ID requested
1	2022-12-13 08:15:36	announced
⏵ 2	2023-11-14 08:55:44	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: CRMPs, TurboID, Toxoplasma gondii,pulldown, apicomplexa

Prof. Dr. rer. nat. Markus Meissner
contact affiliation	Department of Experimental Parasitology LMU München Lena-Christ-Str. 48 82152 Planegg-Martinsried Telefon: +49 (0) 89 2180 - 3622 Fax: +49 (0) 89 2180 - 3623 E-Mail: markus.meissner@lmu.de
contact email	Markus.Meissner@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD031649

PRIDE project URI

• PRIDE
  1. PXD031649
     1. Label: PRIDE project
     2. Name: Identification of interaction partners of the Toxoplasma gondii Cysteine Repeat Modular Proteins TGGT1_292020 and TGGT1_261080