⮝ Full datasets listing

PXD031623

PXD031623 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic and Antibody Profiles Reveals Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19
DescriptionBrucellosis is an important zoonotic disease that causes great economic losses. Vaccine immunisation is the main strategy for the prevention and control of Brucellosis. Although live attenuated vaccines play important roles in the prevention of this disease, they also have several limitations, such as residual virulence and difficulty in the differentiation of immunisation and infection. We developed and evaluated a new bacteria ghost vaccine of Brucella abortus A19 by a new double inactivation method. The results showed that the bacterial ghost vaccine of Brucella represents a more safe and efficient vaccine for Brucellosis. We further characterised the antigenic components and signatures of the vaccine candidate A19BG. Here, we utilised a mass spectrometry-based label-free relative quantitative proteomics approach to investigate the global proteomics changes in A19BGs compared to its parental A19. The proteomic analysis identified 2014 proteins, 1116 of which were differentially expressed compared with those in A19. The common immunological proteins of OMPs (Bcsp31, Omp25, Omp10, Omp19, Omp28, and Omp2a), HSPs (DnaK, GroS, and GroL), and SodC were enriched in the proteome of A19BG. By protein micro array- based antibody profiling, significant differences were observed between A19BG and A19, and a number of signature immunogenic proteins were identified. Two of these proteins, BMEII0032 and BMEI0892, were confirmed to be differential diagnostic antigens for the A19BG vaccine candidate. In conclusion, using comparative proteomics and antibody profiling, protein components and signature antigens were identified for the ghost vaccine candidate A19BG, which are valuable for further developing the vaccine and its monitoring assays.
HostingRepositoryPRIDE
AnnounceDate2022-05-20
AnnouncementXMLSubmission_2022-05-20_06:42:05.356.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterhe chuanyu
SpeciesList scientific name: Brucella abortus; NCBI TaxID: 235;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-11 06:38:22ID requested
12022-05-20 06:42:06announced
Publication List
He CY, Yang JH, Ye YB, Zhao HL, Liu MZ, Yang QL, Liu BS, He S, Chen ZL, A19. Front Immunol, 13():874871(2022) [pubmed]
Keyword List
submitter keyword: Brucellosis, bacteria ghost, vaccine, comparative proteomics, antibody profiling
Contact List
Chuanyu he
contact affiliationTecon Biological Co., Ltd.
contact emailhcy061120@163.com
lab head
he chuanyu
contact affiliationTecon Biological Co., Ltd.
contact emailhcy061120@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031623
PRIDE project URI
Repository Record List
[ + ]