PXD031623

PXD031623 is an original dataset announced via ProteomeXchange.

Title	Proteomic and Antibody Profiles Reveals Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19
Description	Brucellosis is an important zoonotic disease that causes great economic losses. Vaccine immunisation is the main strategy for the prevention and control of Brucellosis. Although live attenuated vaccines play important roles in the prevention of this disease, they also have several limitations, such as residual virulence and difficulty in the differentiation of immunisation and infection. We developed and evaluated a new bacteria ghost vaccine of Brucella abortus A19 by a new double inactivation method. The results showed that the bacterial ghost vaccine of Brucella represents a more safe and efficient vaccine for Brucellosis. We further characterised the antigenic components and signatures of the vaccine candidate A19BG. Here, we utilised a mass spectrometry-based label-free relative quantitative proteomics approach to investigate the global proteomics changes in A19BGs compared to its parental A19. The proteomic analysis identified 2014 proteins, 1116 of which were differentially expressed compared with those in A19. The common immunological proteins of OMPs (Bcsp31, Omp25, Omp10, Omp19, Omp28, and Omp2a), HSPs (DnaK, GroS, and GroL), and SodC were enriched in the proteome of A19BG. By protein micro array- based antibody profiling, significant differences were observed between A19BG and A19, and a number of signature immunogenic proteins were identified. Two of these proteins, BMEII0032 and BMEI0892, were confirmed to be differential diagnostic antigens for the A19BG vaccine candidate. In conclusion, using comparative proteomics and antibody profiling, protein components and signature antigens were identified for the ghost vaccine candidate A19BG, which are valuable for further developing the vaccine and its monitoring assays.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_06:42:05.356.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	he chuanyu
SpeciesList	scientific name: Brucella abortus; NCBI TaxID: 235;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 06:38:22	ID requested
⏵ 1	2022-05-20 06:42:06	announced

He CY, Yang JH, Ye YB, Zhao HL, Liu MZ, Yang QL, Liu BS, He S, Chen ZL, A19. Front Immunol, 13():874871(2022) [pubmed]

submitter keyword: Brucellosis, bacteria ghost, vaccine, comparative proteomics, antibody profiling

Chuanyu he
contact affiliation	Tecon Biological Co., Ltd.
contact email	hcy061120@163.com
lab head
he chuanyu
contact affiliation	Tecon Biological Co., Ltd.
contact email	hcy061120@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031623
     1. Label: PRIDE project
     2. Name: Proteomic and Antibody Profiles Reveals Antigenic Composition and Signatures of Bacterial Ghost Vaccine of Brucella abortus A19