PXD031622

PXD031622 is an original dataset announced via ProteomeXchange.

Title	Skin cancer-associated S. aureus downregulates DNA repair mechanisms, and promotes oxidative stress and DNA damage in human keratinocytes
Description	Actinic keratoses (AK) are premalignant lesions common on photo-damaged skin that, over time, can progress to squamous cell carcinoma (SCC). A high bacterial load of Staphylococcus aureus is associated with AK and SCC but it is unknown whether this has a direct impact on skin cancer development. To determine whether S. aureus is able to trigger pro-tumorigenic skin responses, we performed RNAseq and shotgun proteomics on primary human keratinocytes after challenge with sterile culture supernatant (‘secretome’) from S. aureus clinical strains isolated from AK and SCC. Certain S. aureus secretomes induced keratinocytes to overexpress SCC biomarkers that have been associated with skin carcinogenesis, and upregulate the expression of enzymes linked with reduced skin barrier function. Further, S. aureus secretomes downregulated DNA repair mechanisms and induced oxidative stress markers. Subsequent experiments confirmed that exposure to SCC-derived S. aureus secretomes lead to increased intracellular ROS levels and DNA damage in primary human keratinocytes. Altogether, this study reveal a novel mechanism for the pro-tumorigenic activity of S. aureus. Further studies are required to determine whether S. aureus products promote SCC development in vivo, which would have important implications for the treatment of AK and prevention of SCC.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:20.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Stoll
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 06:38:03	ID requested
1	2022-05-11 06:52:43	announced
2	2022-05-11 08:02:10	announced	2022-05-11: Updated publication reference for DOI(s): doi.org/10.3390/cancers14092143.
⏵ 3	2023-11-14 08:55:21	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: S. aureus secretome, multiomics, actinic keratosis,Skin, DNA damage, squamous cell carcinoma

Michelle M. Hill
contact affiliation	QIMR Berghofer Medical Research Institute, Precision & Systems Biomedicine, Herston, Brisbane, Queensland, Australia
contact email	Michelle.Hill@qimrberghofer.edu.au
lab head
Thomas Stoll
contact affiliation	QIMR Berghofer Medical Research Institute 300 Herston Road, Herston QLD 4006
contact email	thomas.stoll@qimrberghofer.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031622

PRIDE project URI

• PRIDE
  1. PXD031622
     1. Label: PRIDE project
     2. Name: Skin cancer-associated S. aureus downregulates DNA repair mechanisms, and promotes oxidative stress and DNA damage in human keratinocytes