PXD031619

PXD031619 is an original dataset announced via ProteomeXchange.

Title	SILAC immunoprecipitations for rabbit haemorrhagic disease virus (RHDV) proteins p23 and RNA-dependent RNA polymerase
Description	Rabbit haemorrhagic disease virus (RHDV) belongs to the family Caliciviridae, genus Lagovirus and is used in Australia as a biocontrol tool to keep the population of european rabbits low. This virus has a positive-sense single-stranded RNA genome that encodes structural (capsid) and non-structural proteins. Due to the lack of an established cell culture system for this virus, some of the non-structural proteins are yet awaiting characterisation and their function is unknown. This work was aimed at the identification of cellular interactors of RHDV proteins p23 and RNA-dependent RNA polymerase (RdRp). p23 has an unknown function and RdRp is the main enzyme that replicates viral genome. SILAC-labeled rabbit kidney (RK13) cells were transfected with the FLAG-tagged viral proteins and GFP-transfected cells served as an internal control. For example, two replicates of heavy Arg and Lys labeled cells were transfected with protein p23 and two replicates of light Arg and Lys labeled cells were transfected with GFP. For the third replicate this was swapped: 'heavy' cells were transfected with GFP and 'light' cells were transfected with p23. After that, cells were harvested, lysed in mild conditions and applied onto anti-FLAG antibody resin for immunoprecipitation. Eluates from the resin were processed for mass spectrometry processing.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:35:33.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Smertina
SpeciesList	scientific name: Oryctolagus cuniculus cuniculus; NCBI TaxID: 568996;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 06:36:58	ID requested
1	2022-10-14 12:21:00	announced
⏵ 2	2023-11-14 07:35:34	announced	2023-11-14: Updated project metadata.

Smertina E, Carroll AJ, Boileau J, Emmott E, Jenckel M, Vohra H, Rolland V, Hands P, Hayashi J, Neave MJ, Liu JW, Hall RN, Strive T, Frese M, Lagovirus Non-structural Protein p23: A Putative Viroporin That Interacts With Heat Shock Proteins and Uses a Disulfide Bond for Dimerization. Front Microbiol, 13():923256(2022) [pubmed]

submitter keyword: interactors, SILAC, co-IP,RHDV

Tanja Strive
contact affiliation	Group leader
contact email	tanja.strive@csiro.au
lab head
Elena Smertina
contact affiliation	University of Canberra/CSIRO
contact email	elena.smertina@csiro.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD031619

PRIDE project URI

• PRIDE
  1. PXD031619
     1. Label: PRIDE project
     2. Name: SILAC immunoprecipitations for rabbit haemorrhagic disease virus (RHDV) proteins p23 and RNA-dependent RNA polymerase