PXD031603

PXD031603 is an original dataset announced via ProteomeXchange.

Title	Interactomes of GluA1 and GluA3 subunit containing AMPARs reveal distinct protein composition
Description	The AMPA glutamate receptor (AMPAR) is the major type of synaptic excitatory ionotropic receptor in the brain. The most abundant AMPAR subtypes in the hippocampus are GluA1/2 and GluA2/3 heterotetramers. Each subtype contributes differentially to mechanisms of synaptic plasticity, which may be in part caused by how these receptors are regulated by specific associated proteins. A broad range of AMPAR interacting proteins have been identified and several were shown to affect biogenesis, AMPAR trafficking, and channel properties, alone or in distinct assemblies, and several revealed preferred binding to specific AMPAR subunits. To date, a systematic separate interactome analysis of the major GluA1/2 and GluA2/3 AMPAR subtypes is lacking. To reveal interactors belonging to specific AMPAR sub-complexes, we performed both quantitative and interaction proteomics on hippocampi of wildtype and GluA1- or GluA3 knock-out mice. Whereas GluA1/2 receptors co-purified TARP-γ8, PRRT1 and CNIH2 with highest abundances, GluA2/3 receptors revealed strongest co-purification of CNIH2, TARP-γ2, and OLFM1. Further analysis revealed that TARP-γ8-PRRT1 can interact directly, and co-assemble into an AMPAR subcomplex especially near the synapse.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:17:04.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie van der Spek
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidoethyl-L-cysteine; L-cysteine methyl disulfide
Instrument	LTQ Orbitrap Discovery; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 06:12:56	ID requested
⏵ 1	2024-10-08 11:17:05	announced

van der Spek SJF, Pandya NJ, Koopmans F, Paliukhovich I, van der Schors RC, Otten M, Smit AB, Li KW, Expression and Interaction Proteomics of GluA1- and GluA3-Subunit-Containing AMPARs Reveal Distinct Protein Composition. Cells, 11(22):(2022) [pubmed]

10.3390/cells11223648;

submitter keyword: proteomics, PRRT1, GluA1/2, TARP- γ8, subcomplexes,AMPAR, GluA2/3, MS-DAP

Ka Wan Li
contact affiliation	Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands
contact email	k.w.li@vu.nl
lab head
Sophie van der Spek
contact affiliation	CNCR, VU Amsterdam
contact email	sophiejf@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD031603

PRIDE project URI

• PRIDE
  1. PXD031603
     1. Label: PRIDE project
     2. Name: Interactomes of GluA1 and GluA3 subunit containing AMPARs reveal distinct protein composition