PXD031600
PXD031600 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of linear ubiquitination substrates based on NAEK-Ub probe in live cells |
Description | While linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination, the none-specific binding of linear polyubiquitin chain, and the high background interferences arising from heterogeneous ubiquitin linkages (e.g. K48-, K63-). We developed a bioorthogonal linear ubiquitin probe by site-specific encoding of a norbornene amino acid (NAEK) on ubiquitin. This probe facilitates covalent labeling of linear ubiquitin substrates in live cells and enables selective enrichment and identification of linear-ubiquitin modified proteins. we applied this probe in the profiling of substrates of linear ubiquitination in HEK293T cells and GSCs. |
HostingRepository | iProX |
AnnounceDate | 2022-02-11 |
AnnouncementXML | Submission_2023-04-19_22:03:40.761.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xianli Du |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-02-11 00:38:57 | ID requested | |
⏵ 1 | 2023-04-19 22:03:41 | announced |
Publication List
Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J, Wu N, Man J, Li H, Li A, Zhang T, Wang B, Duan X, A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma. Nucleic Acids Res, 51(3):1050-1066(2023) [pubmed] |
Keyword List
submitter keyword: Linear ubiquitination, bio-orthogonal probe, NAEK-Ub, live cells |
Contact List
Xiaotao Duan | |
---|---|
contact affiliation | Beijing Institute of Pharmacology and Toxicology |
contact email | duan_xt@hotmail.com |
lab head | |
Xianli Du | |
contact affiliation | Beijing Institute of Pharmacology and Toxicology |
contact email | dxlxp@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |