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PXD031600

PXD031600 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of linear ubiquitination substrates based on NAEK-Ub probe in live cells
DescriptionWhile linear ubiquitin plays critical roles in multiple cell signaling pathways, few substrates have been identified. Global profiling of linear ubiquitin substrates represents a significant challenge because of the low endogenous level of linear ubiquitination, the none-specific binding of linear polyubiquitin chain, and the high background interferences arising from heterogeneous ubiquitin linkages (e.g. K48-, K63-). We developed a bioorthogonal linear ubiquitin probe by site-specific encoding of a norbornene amino acid (NAEK) on ubiquitin. This probe facilitates covalent labeling of linear ubiquitin substrates in live cells and enables selective enrichment and identification of linear-ubiquitin modified proteins. we applied this probe in the profiling of substrates of linear ubiquitination in HEK293T cells and GSCs.
HostingRepositoryiProX
AnnounceDate2022-02-11
AnnouncementXMLSubmission_2023-04-19_22:03:40.761.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXianli Du
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-11 00:38:57ID requested
12023-04-19 22:03:41announced
Publication List
Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J, Wu N, Man J, Li H, Li A, Zhang T, Wang B, Duan X, A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma. Nucleic Acids Res, 51(3):1050-1066(2023) [pubmed]
Keyword List
submitter keyword: Linear ubiquitination, bio-orthogonal probe, NAEK-Ub, live cells
Contact List
Xiaotao Duan
contact affiliationBeijing Institute of Pharmacology and Toxicology
contact emailduan_xt@hotmail.com
lab head
Xianli Du
contact affiliationBeijing Institute of Pharmacology and Toxicology
contact emaildxlxp@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI