PXD031597

PXD031597 is an original dataset announced via ProteomeXchange.

Title	Label-free Quantitative Proteomic analysis of the mechanism of salt stress promoting selenium enrichment in Lactobacillus rhamnosus
Description	Lactobacillus rhamnosus can metabolize selenite into organic selenium and Se0. In this paper, label-free quantitative proteomics was applied to explore the mechanism of salt stress promoting selenium enrichment of L.rhamnosus. 397 proteins were up-regulated and 147 proteins were down-regulated of selenium-enriched L.rhamnosus under salt stress. The differentially expressed proteins (DEPs) were mainly involved in metabolism, membrane transport and genetic information processing. The results of quantitative real-time PCR showed that gene opuA, metN, trxR and ldh of Se-enriched L.rhamnosus with salt sress were significantly up-regulated. However, the expression levels of gene luxS, groEL, dnaK and pgk were down-regulated. It was indicated that L.rhamnosus promoted part of amino acids combining with selenium into selenoamino acids with salt stress. Secondly, sodium chloride stimulated the expression of key enzymes involved in metabolism to provide energy for the process of Se-enrichment. In addition, NaCl induced the expression of enzymes and genes involved in the synthesis of selenoproteins.
HostingRepository	iProX
AnnounceDate	2022-02-11
AnnouncementXML	Submission_2023-12-24_20:37:21.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ying Xu
SpeciesList	scientific name: Lactobacillus rhamnosus ATCC 21052; NCBI TaxID: 1002365;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-10 21:24:22	ID requested
⏵ 1	2023-12-24 20:37:22	announced

Xu Y, Wu S, Wang P, Wei L, Li H, Label-free quantitative proteomic analysis of the mechanism of salt stress promoting selenium enrichment in Lactobacillus rhamnosus. J Proteomics, 265():104663(2022) [pubmed]

submitter keyword: Selenium, Lactobacillus rhamnosus, proteomics, gene, mechanism

Hongliang Li
contact affiliation	Shaanxi University of Science and Technology
contact email	lihongliang@sust.edu.cn
lab head
Ying Xu
contact affiliation	Shaanxi University of Science and Technology
contact email	xuying@sust.edu.cn
dataset submitter

iProX dataset URI