PXD031596

PXD031596 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of key organs reveal the significant roles of antioxidant enzymes and oxidative phosphorylation proteins in starved ticks Haemaphysalis longicornis Neumann
Description	Haemaphysalis longicornis Neumann, a tick of public health and veterinary importance, spend the major part of their life cycle off-host, especially the adult host-seeking period. Thus, they have to contend with prolonged starvation (sometimes for an extended period of 1-2 years). Here, we investigated the underlying molecular mechanism of tick starvation endurance in the salivary gland, midgut, ovary, and Malpighian tubule of starved H. longicornis ticks using the data-independent acquisition (DIA) quantitative proteomic approach to study the proteome changes. “Ribosome” was the most enriched KEGG pathway with the participation of more than thirty differentially expressed ribosomal proteins, indicating ribosomal stress. Essential synthases such as glutamate synthase, citrate synthase, ATP synthase, and ATP-citrate synthase were upregulated probably due to increased proteolysis and amino acid catabolism during starvation. The upregulation of succinate dehydrogenase, ATP synthase, cytochrome c oxidase, and ADP/ATP translocase closely fits with an increased oxidative phosphorylation function during starvation. The differential expression of superoxide dismutase, glutathione reductase, glutathione S-transferase, thioredoxin, thioredoxin reductase, and peroxiredoxin indicated fasting-induced oxidative stress. The upregulation of heat shock proteins could imply the activation of a protective mechanism that checks excessive protein breakdown during starvation stress. The survival of the ticks reduced after silencing of target genes via RNAi but was not significant relative to GFP-dsRNA injected ticks. The results of this study could provide useful information about the vulnerabilities of ticks that could aid in tick control efforts.
HostingRepository	iProX
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-10-08_21:09:39.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ningmei Wang
SpeciesList	scientific name: Haemaphysalis longicornis; NCBI TaxID: 44386;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-10 19:07:04	ID requested
1	2022-02-10 19:09:53	announced
⏵ 2	2022-10-08 21:09:40	announced	2022-10-09: Update publication information.

Wang N, Wang H, Ji A, Li N, Chang G, Liu J, Agwunobi DO, Wang H, Proteomic changes in various organs of Haemaphysalis longicornis under long-term starvation. PLoS Negl Trop Dis, 16(8):e0010692(2022) [pubmed]

submitter keyword: adaptation, hunger stress, oxidative stress, quantitative proteomics

Hui Wang
contact affiliation	Hebei Normal University
contact email	whui1981@163.com
lab head
Ningmei Wang
contact affiliation	Hebei Normal University
contact email	767247571@qq.com
dataset submitter

iProX dataset URI