PXD031591

PXD031591 is an original dataset announced via ProteomeXchange.

Title	Identification of Plin5 binding partners involved in lipid droplet-mitochondrial contacts
Description	Perilipin 5 (Plin5) has been shown the induce contact between mitochondria and lipid droplets, though the mechanism by which this occurs is unknown. It has been shown that the unique C-terminal domain of Plin5 is responsible for inducing these contact sites. In this proteomics study, we identify binding partners which mediate this function by performing affinity purification-mass spectrometry using GFP-tagged Plin5 constructs where Plin5 is full length, lacking half of the c-terminal domain, or lacking the entire c-terminal domain. Of the several potential interactors, we validate Fatp4 as a direct interactor with the tether domain of Plin5.
HostingRepository	PRIDE
AnnounceDate	2023-04-06
AnnouncementXML	Submission_2023-04-06_13:38:29.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LauraHerring
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-10 15:13:50	ID requested
⏵ 1	2023-04-06 13:38:29	announced

Dataset with its publication pending

submitter keyword: metabolism, Plin5,AP-MS

SarahCohen
contact affiliation	Department of Cell Biology and Physiology, UNC-Chapel Hill
contact email	sarahcoh@ad.unc.edu
lab head
LauraHerring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD031591

PRIDE project URI

• PRIDE
  1. PXD031591
     1. Label: PRIDE project
     2. Name: Identification of Plin5 binding partners involved in lipid droplet-mitochondrial contacts