PXD031565

PXD031565 is an original dataset announced via ProteomeXchange.

Title	Extraction of A. fumigatus proteins from culture-filtrate-exposed fungal cultures.
Description	The fungal pathogen Aspergillus fumigatus is frequently cultured from the sputum of cystic fibrosis (CF) patients along with the bacterium, Pseudomonas aeruginosa. A. fumigatus secretes a range of secondary metabolites, and one of these, gliotoxin, has inhibitory effects on the host immune response. In this study, the effect of P. aeruginosa culture filtrate (CuF) on fungal growth and gliotoxin production was investigated. Exposure of A. fumigatus hyphae to P. aeruginosa cells induced increased production of gliotoxin and a decrease in fungal growth. In contrast exposure of A. fumigatus hyphae to P. aeruginosa CuF lead to increased growth and decreased gliotoxin production. Quantitative proteomic analysis was employed to characterize the proteomic response of A. fumigatus upon exposure to P. aeruginosa CuF. Changes in the profile of proteins involved with secondary metabolite biosynthesis (gliotoxin, fumagillin, pseurotin A), and changes to the abundance of proteins involved in oxidative stress (e.g. formate dehydrogenase) and detoxification (e.g. thioredoxin reductase) were observed, suggesting that the bacterial secretome has a profound effect on the fungal proteome. Alterations in the abundance of proteins involved in detoxification and oxidative stress, highlight the ability of A. fumigatus to differentially regulate protein synthesis in response to environmental stresses imposed by competitors such as P. aeruginosa. Such responses may ultimately have serious detrimental effects on the host.
HostingRepository	PRIDE
AnnounceDate	2023-03-11
AnnouncementXML	Submission_2023-03-11_02:12:04.963.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JamesCarolan
SpeciesList	scientific name: Pseudomonas aeruginosa group; NCBI TaxID: 136841;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 12:25:37	ID requested
⏵ 1	2023-03-11 02:12:05	announced

Margalit A, Sheehan D, Carolan JC, Kavanagh K, . Microbiology (Reading), 168(3):(2022) [pubmed]

submitter keyword: culture filtrate, LFQ proteomics,Aspergillus fumigatus, Pseudomonas aeruginosa

JimCarolan
contact affiliation	Applied Proteomics Lab, Department of Biology, Maynooth University, Ireland.
contact email	james.carolan@nuim.ie
lab head
JamesCarolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031565
     1. Label: PRIDE project
     2. Name: Extraction of A. fumigatus proteins from culture-filtrate-exposed fungal cultures.