PXD031563

PXD031563 is an original dataset announced via ProteomeXchange.

Title	Investigating the upstream signaling of Imh1 phosphorylation under ER stress in yeast
Description	The accumulation of misfolded proteins in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR), which acts through various mechanisms to reduce ER stress. We previously found ER stress induced by tunicamycin (TM) treatment promotes the activation of small GTPase Arl1 and thereby increasing the recruitment of downstream effector golgin Imh1 to the late-Golgi. However, the role of Imh1 under ER stress remains unknown. In this study, we found Imh1 is required for the recycling of two SNAREs, Snc1 and Tlg1, upon TM-induced ER stress and phosphorylation of Imh1 is required for this regulation. Since Ire1 is the sensor of UPR in yeast and we previous found that Ire1 is required for Arl1-Imh1 activation, we wonder whether Ire1 signaling is also responsible for the TM-induced phosphorylation of Imh1 and the subsequent SNARE transport. We compared the phosphorylation signal of Imh1 in WT with that in the absence of Ire1 and further performed SILAC method in a label swap replication. Collectively, this study illustrates the mechanism of how signaling under ER stress promotes Imh1 in cooperation with another tether factor in regulating the SNARE transport to alleviate ER stress in yeast cells.
HostingRepository	PRIDE
AnnounceDate	2022-07-21
AnnouncementXML	Submission_2022-07-21_05:55:35.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 12:23:48	ID requested
⏵ 1	2022-07-21 05:55:37	announced

Wang YH, Chiu WY, Chen YT, Cai PJ, Wu YC, Wu JL, Chen BH, Liu YW, Yu CJ, Lee FS, Golgin Imh1 and GARP complex cooperate to restore the impaired SNARE recycling transport induced by ER stress. Cell Rep, 38(12):110488(2022) [pubmed]

submitter keyword: GTPase Arl1, Imh1, ER stress, phosphorylation, Ire1, SNARE transport

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
lab head
Chia-Jung Yu
contact affiliation	259 Wen-Hwa 1st Road, Guishan District, Tao-Yuan City, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031563
     1. Label: PRIDE project
     2. Name: Investigating the upstream signaling of Imh1 phosphorylation under ER stress in yeast