PXD031562

PXD031562 is an original dataset announced via ProteomeXchange.

Title	Identification of Golgin Imh1 phosphorylation residues under ER stress in yeast
Description	The accumulation of misfolded proteins in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR), which acts through various mechanisms to reduce ER stress. We previously found ER stress induced by tunicamycin (TM) treatment promotes the activation of small GTPase Arl1 and thereby increasing the recruitment of downstream effector golgin Imh1 to the late-Golgi. However, the role of Imh1 under ER stress remains unknown. In this study, we found Imh1 is required for the recycling of two SNAREs, Snc1 and Tlg1, upon TM-induced ER stress. Due to Imh1 is already known as phosphorylation protein, we wonder whether phosphorylation play roles in regulating the function of Imh1 under ER stress. We utilized SILAC method to identify the phosphorylation sites on Imh1 upon TM treatment through MS analysis and found several sites increased phosphorylation under ER stress. We finally selected the predominant S25/T27 phosphorylated residues and study the mechanism of how S25/T27 phosphorylation regulates the function of Imh1 in SNARE transport upon TM treatment.
HostingRepository	PRIDE
AnnounceDate	2022-07-21
AnnouncementXML	Submission_2022-07-21_05:56:29.542.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 12:23:28	ID requested
⏵ 1	2022-07-21 05:56:30	announced

Wang YH, Chiu WY, Chen YT, Cai PJ, Wu YC, Wu JL, Chen BH, Liu YW, Yu CJ, Lee FS, Golgin Imh1 and GARP complex cooperate to restore the impaired SNARE recycling transport induced by ER stress. Cell Rep, 38(12):110488(2022) [pubmed]

submitter keyword: GTPase Arl1, Imh1, ER stress, phosphorylation, SNARE transport

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
lab head
Chia-Jung Yu
contact affiliation	259 Wen-Hwa 1st Road, Guishan District, Tao-Yuan City, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031562
     1. Label: PRIDE project
     2. Name: Identification of Golgin Imh1 phosphorylation residues under ER stress in yeast