PXD031526

PXD031526 is an original dataset announced via ProteomeXchange.

Title	Systematic Identification of the Lysine lactylation in the Protozoan Parasite Toxoplasma gondii
Description	Lysine lactylation (Kla) is a new posttranslational modification (PTM) identified in histone and nonhistone proteins of several eukaryotic cells that directly activate gene expression and DNA replication. However, very little is known about their scope and cellular distribution in apicomplexan parasites that are important to public and animal health. Toxoplasma gondii, the agent of toxoplasmosis, is one of obligate intracellular apicomplexan parasite that can infect all kinds of nucleated cells of animals and humans. Using this parasite as model organism, herein, we produced the first global lysine lactylome profile through LC-MS/MS. Overall, a total of 983 Kla sites occurred on 523 lactylated proteins were identified in Toxoplasma tachyzoites, the acute toxoplasmosis-causing stage. Bioinformatics analysis revealed that these lactylated proteins are evolutionarily conserved and involved in a wide variety of cellular functions such as energy metabolism, gene regulation and protein biosynthesis. Moreover, the results from subcellular localization analysis and IFA showed that the majority of lactylated T. gondii proteins localized in the nucleus, indicating a potential impact of Kla on gene regulation. Notably, an extensive batch of parasite-specific proteins unique to Apicomplexa is lactylated in T. gondii. Our findings revealed that Kla was widespread in the early branching eukaryotic cell, and that lactylated proteins, including a crowd of unique parasite proteins, were involved in a remarkably diverse array of cellular functions. These valuable data will improve our understanding of the evolution of Kla while potentially providing novel therapeutic avenues.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_04:04:10.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Tan
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 05:09:45	ID requested
⏵ 1	2022-06-09 04:04:11	announced

Zhao W, Yu H, Liu X, Wang T, Yao Y, Zhou Q, Zheng X, Tan F, Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii. Parasit Vectors, 15(1):180(2022) [pubmed]

submitter keyword: Toxoplasma gondii,lysine lactylation,LC-MSMS

Feng Tan
contact affiliation	Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China
contact email	tanfengsong@163.com
lab head
Feng Tan
contact affiliation	Wenzhou Medical University
contact email	tanfengsong@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031526
     1. Label: PRIDE project
     2. Name: Systematic Identification of the Lysine lactylation in the Protozoan Parasite Toxoplasma gondii