PXD031519

PXD031519 is an original dataset announced via ProteomeXchange.

Title	Cross-linking mass spectrometry and integrative modelling of Methanocaldococcus jannaschii GMP synthetase
Description	GMP synthetases are enzymes that catalyze the conversion of XMP to GMP. The two-subunit type GMP synthetases are composed of a glutamine amidotransferase (GATase) subunit that catalyzes the conversion of Gln to Glu and ammonia, and the ATP pyrophosphatase (ATPPase) subunit that catalyzes the formation of AMP-XMP from ATP and XMP. The inactive GATase subunit is allosterically activated by the binding of substrates to the ATPPase subunit. Upon activation, the GATase subunit binds Gln and hydrolyzes it producing ammonia which is tunnelled to the ATPPase subunit. The two subunits form a tight complex to enable domain crosstalk. However, the Methanocaldococcus jannaschii GMP synthetase (MjGMPS) is unique as the GATase (MjGATase) and ATPPase (MjATPPase) subunits interact transiently. Here, we employed enzyme kinetics, X-ray crystallography, cross-linking mass spectrometry (XL-MS) and integrative modelling to understand the mechanistic basis for the various steps in the catalytic cycle of MjGMPS.
HostingRepository	PRIDE
AnnounceDate	2022-09-02
AnnouncementXML	Submission_2022-09-01_23:57:05.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Santosh Shivakumaraswamy
SpeciesList	scientific name: Methanocaldococcus jannaschii DSM 2661; NCBI TaxID: 243232;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 03:45:59	ID requested
⏵ 1	2022-09-01 23:57:06	announced

Shivakumaraswamy S, Kumar S, Bellur A, Polisetty SD, Balaram H, Mechanistic Insights into the Functioning of a Two-Subunit GMP Synthetase, an Allosterically Regulated, Ammonia Channeling Enzyme. Biochemistry, 61(18):1988-2006(2022) [pubmed]

submitter keyword: GMP synthetase, allostery, ammonia tunnelling, cross-linking mass spectrometry, integrative modelling

Hemalatha Balaram
contact affiliation	Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bengaluru 560064, India.
contact email	hb@jncasr.ac.in
lab head
Santosh Shivakumaraswamy
contact affiliation	Molecular Biology and Genetics Unit, JNCASR
contact email	porustg@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031519
     1. Label: PRIDE project
     2. Name: Cross-linking mass spectrometry and integrative modelling of Methanocaldococcus jannaschii GMP synthetase