PXD031516

PXD031516 is an original dataset announced via ProteomeXchange.

Title	Mouse kidney and cell lines LC-MS/MS
Description	Zinc (Zn) is an essential micronutrient and cofactor for up to 10% of proteins in living organisms. During Zn limitation, specialized enzymes called metallochaperones are predicted to allocate Zn to specific metalloproteins. This function has been putatively assigned to G3E GTPase COG0523 proteins, yet no Zn metallochaperone has been experimentally identified in any organism. Here, we functionally characterize a family of COG0523 proteins that is conserved across vertebrates. We identify Zn metalloprotease METAP1 as a COG0523 client, leading to the redesignation of this group of COG0523 proteins as Zn regulated GTPase metalloprotein activator (ZNG1) family. Using biochemical, genetic, structural, and pharmacological approaches across evolutionarily divergent models, including zebrafish and mice, we demonstrate a critical role for ZNG1 proteins in regulating cellular Zn homeostasis. Collectively, these data reveal the existence of a family of Zn metallochaperones and place ZNG1 at the center of a new paradigm for intracellular Zn trafficking.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:03:02.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angela Kruse
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 03:24:47	ID requested
1	2023-07-20 11:12:36	announced
⏵ 2	2023-11-14 09:03:05	announced	2023-11-14: Updated project metadata.

Weiss A, Murdoch CC, Edmonds KA, Jordan MR, Monteith AJ, Perera YR, Rodr, í, guez Nassif AM, Petoletti AM, Beavers WN, Munneke MJ, Drury SL, Krystofiak ES, Thalluri K, Wu H, Kruse ARS, DiMarchi RD, Caprioli RM, Spraggins JM, Chazin WJ, Giedroc DP, Skaar EP, Zn-regulated GTPase metalloprotein activator 1 modulates vertebrate zinc homeostasis. Cell, 185(12):2148-2163.e27(2022) [pubmed]

submitter keyword: mouse, targeteed, shotgun, zinc

Eric P. Skaar
contact affiliation	Department of Pathology, Microbiology, and Immunology;Vanderbilt Institute for Infection, Immunology, and Inflammation. Vanderbilt University Medical Center, Nashville, TN USA
contact email	eric.skaar@vumc.org
lab head
Angela Kruse
contact affiliation	Vanderbilt University
contact email	ark253@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD031516

PRIDE project URI

• PRIDE
  1. PXD031516
     1. Label: PRIDE project
     2. Name: Mouse kidney and cell lines LC-MS/MS