PXD031515

PXD031515 is an original dataset announced via ProteomeXchange.

Title	GPR143 regulates ESCRT-dependent exosome biogenesis and promotes cancer metastasis
Description	Exosomes transport a variety of macromolecules and modulate intercellular communication in physiology and disease. However, the regulation mechanisms that determine exosome contents during exosome biogenesis remain poorly understood. Here, we identify GPR143, an atypical GPCR, as a regulator of the endosomal sorting complex required for transport (ESCRT)-dependent exosome biogenesis pathway. GPR143 interacts with HRS (an ESCRT-0 Subunit) and promotes its association to cargo proteins, such as EGFR, which subsequently enables selective protein sorting into intralumenal vesicles (ILVs) in multivesicular bodies (MVBs). GPR143 is elevated in multiple cancers and quantitative proteomic and RNA profiling of exosomes revealed the GPR143-ESCRT pathway promotes secretion of exosomes that carry unique cargo, including integrins signaling proteins. By gain- and loss-of-function studies, we reveal GPR143 promotes metastasis by secreting exosomes and increasing cell motility/invasion through the integrin/FAK/Src pathway. These finding uncover a mechanism that regulates the exosomal proteome and demonstrate its ability to promote cancer metastasis.
HostingRepository	PRIDE
AnnounceDate	2022-12-20
AnnouncementXML	Submission_2022-12-20_08:10:18.430.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	YU JINLEE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-09 03:22:28	ID requested
⏵ 1	2022-12-20 08:10:18	announced

10.6019/PXD031515;

submitter keyword: breast cancer, Exosomal protein sorting, Melanoma,GPR143, HRS, MVB

Young ChanChae
contact affiliation	Department of Biological Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 44919, Republic of Korea
contact email	ychae@unist.ac.k
lab head
YU JINLEE
contact affiliation	UNIST
contact email	yjlee0627@unist.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031515
     1. Label: PRIDE project
     2. Name: GPR143 regulates ESCRT-dependent exosome biogenesis and promotes cancer metastasis