PXD031497

PXD031497 is an original dataset announced via ProteomeXchange.

Title	The rice aspartyl-tRNA synthetase YLC3 regulates amino acid homeostasis and chloroplast development under low temperature
Description	Aminoacyl tRNA synthetases are key enzymes in protein synthesis, attaching the proper amino acid to the corresponding tRNA to make protein. However, their roles in regulating plant growth and development still remain elusive. Here we reported a rice thermo-sensitive mutant yellow leaf chlorosis3 (ylc3) with reduced chlorophyll content, altered thylakoid structure, and substantially elevated levels of free aspartate, asparagine and glutamine in leaves under low temperature condition. Map-based cloning identified that YLC3 encodes an aspartyl-tRNA synthetase which is localized in cytosol and mitochondria. In addition, quantitative proteomics analysis revealed that both nuclear and chloroplast-encoded thylakoid proteins were significantly down-regulated in the mutant. On the other hand, proteins involved in amino acid metabolism and the process of protein synthesis were up-regulated in ylc3, particularly for key enzymes that convert aspartate to asparagine. Moreover, uncharged tRNA-Asp accumulation and the phosphorylation of rice translation initiation factor eIF2α(the alpha subunit of eukaryotic translation initiation factor 2) is detected , suggesting that YLC3 regulates the homeostasis of amino acid metabolism and chloroplast thylakoid development through regulation of the protein translation and synthesis processes.
HostingRepository	iProX
AnnounceDate	2022-02-08
AnnouncementXML	Submission_2023-02-02_19:03:38.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiping Deng
SpeciesList	scientific name: Oryza sativa; NCBI TaxID: 4530;
ModificationList	TMT6plex reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-08 00:13:54	ID requested
1	2022-02-08 00:14:14	announced
⏵ 2	2023-02-02 19:03:38	announced	2023-02-03: Update publication information.

Liu H, Gong X, Deng H, Tan J, Sun Y, Wang F, Wu W, Zhou Z, Xu R, He H, Lo C, The Rice Aspartyl-tRNA Synthetase YLC3 Regulates Amino Acid Homeostasis and Chloroplast Development Under Low Temperature. Front Plant Sci, 13():847364(2022) [pubmed]

submitter keyword: rice, aspartyl-tRNA synthetase, amino acid homeostasis, chloroplast development, low temperature

Hongjia Liu
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	liuhongjia@zaas.ac.cn
lab head
Zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
dataset submitter

iProX dataset URI