PXD031484

PXD031484 is an original dataset announced via ProteomeXchange.

Title	A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides
Description	C-mannosylated peptides are easily detected by mass spectrometry (MS) from purified proteins but not from complex biological samples. Enrichment of specific glycopeptides by lectin affinity prior to MS analysis has been widely applied to support glycopeptide identification, but was up till now not available for C-mannosylated peptides. Here we used the α-mannose-specific Burkholderia cenocepacia lectin A (BC2L-A) and show that, in addition to its previously demonstrated high-mannose N-glycan binding capability, this lectin is able to retain C- and O-mannosylated peptides. We evaluated the binding abilities of BC2L-A to standard peptides. In conclusion, BC2L-A enabled specific enrichment of C- and O-mannosylated peptides and might have superior properties over other lectins for binding to α-mannose-terminating glycans.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_03:03:58.647.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valerian Grote
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	carbamoylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-07 07:36:01	ID requested
⏵ 1	2022-05-31 03:03:59	announced

H, ü, tte HJ, Tiemann B, Shcherbakova A, Grote V, Hoffmann M, Povolo L, Lommel M, Strahl S, Vakhrushev SY, Rapp E, Buettner FFR, Halim A, Imberty A, Bakker H, A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C- and O-Mannosylated Peptides. Anal Chem, 94(20):7329-7338(2022) [pubmed]

submitter keyword: LC-MSMS, proteomics, lectin, C-mannosylation, O-mannosylation, N-linked glycosylation, glycosylation

Marcus Hoffmann
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems Bioprocess Engineering Bio/Process Analytics
contact email	mhoffmann@mpi-magdeburg.mpg.de
lab head
Valerian Grote
contact affiliation	Max Planck Institute for Dynamics of Complex Technical Systems Bioprocess Engineering Bio/Process Analytics
contact email	grote@mpi-magdeburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031484

PRIDE project URI

• PRIDE
  1. PXD031484
     1. Label: PRIDE project
     2. Name: A Bacterial Mannose Binding Lectin as a Tool for the Enrichment of C-and O- Mannosylated Peptides