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PXD031468

PXD031468 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUbiquitin-based pathway acts inside chloroplasts to regulate photosynthesis
DescriptionPhotosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated, and processed via the CHLORAD pathway: they are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically-derived chloroplasts, where it acts to regulate one of the most fundamental processes of life.
HostingRepositoryiProX
AnnounceDate2022-01-31
AnnouncementXMLSubmission_2022-11-20_22:41:21.319.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZujie Yao
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListubiquitination signature dipeptidyl lysine
InstrumenttimsTOF Pro; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-06 00:41:27ID requested
12022-11-15 20:24:15announced
22022-11-20 22:41:22announced2022-11-21: Update publication information.
Publication List
Sun Y, Yao Z, Ye Y, Fang J, Chen H, Lyu Y, Broad W, Fournier M, Chen G, Hu Y, Mohammed S, Ling Q, Jarvis RP, Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis. Sci Adv, 8(46):eabq7352(2022) [pubmed]
Keyword List
submitter keyword: chloroplasts, CHLORAD, organelle, proteolysis, ubiquitin, ubiquitinom
Contact List
Qihua Ling
contact affiliationNational Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
contact emailqhling@cemps.ac.cn
lab head
Zujie Yao
contact affiliationNational Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
contact emailzjyao@cemps.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI