PXD031468

PXD031468 is an original dataset announced via ProteomeXchange.

Title	Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis
Description	Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated, and processed via the CHLORAD pathway: they are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically-derived chloroplasts, where it acts to regulate one of the most fundamental processes of life.
HostingRepository	iProX
AnnounceDate	2022-01-31
AnnouncementXML	Submission_2022-11-20_22:41:21.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zujie Yao
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	timsTOF Pro; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-06 00:41:27	ID requested
1	2022-11-15 20:24:15	announced
⏵ 2	2022-11-20 22:41:22	announced	2022-11-21: Update publication information.

Sun Y, Yao Z, Ye Y, Fang J, Chen H, Lyu Y, Broad W, Fournier M, Chen G, Hu Y, Mohammed S, Ling Q, Jarvis RP, Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis. Sci Adv, 8(46):eabq7352(2022) [pubmed]

submitter keyword: chloroplasts, CHLORAD, organelle, proteolysis, ubiquitin, ubiquitinom

Qihua Ling
contact affiliation	National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
contact email	qhling@cemps.ac.cn
lab head
Zujie Yao
contact affiliation	National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
contact email	zjyao@cemps.ac.cn
dataset submitter

iProX dataset URI