PXD031468
PXD031468 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis |
Description | Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated, and processed via the CHLORAD pathway: they are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically-derived chloroplasts, where it acts to regulate one of the most fundamental processes of life. |
HostingRepository | iProX |
AnnounceDate | 2022-01-31 |
AnnouncementXML | Submission_2022-11-20_22:41:21.319.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zujie Yao |
SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | timsTOF Pro; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-02-06 00:41:27 | ID requested | |
1 | 2022-11-15 20:24:15 | announced | |
⏵ 2 | 2022-11-20 22:41:22 | announced | 2022-11-21: Update publication information. |
Publication List
Sun Y, Yao Z, Ye Y, Fang J, Chen H, Lyu Y, Broad W, Fournier M, Chen G, Hu Y, Mohammed S, Ling Q, Jarvis RP, Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis. Sci Adv, 8(46):eabq7352(2022) [pubmed] |
Keyword List
submitter keyword: chloroplasts, CHLORAD, organelle, proteolysis, ubiquitin, ubiquitinom |
Contact List
Qihua Ling | |
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contact affiliation | National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences |
contact email | qhling@cemps.ac.cn |
lab head | |
Zujie Yao | |
contact affiliation | National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences |
contact email | zjyao@cemps.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |