PXD031463

PXD031463 is an original dataset announced via ProteomeXchange.

Title	The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts
Description	Sporozoites and merozoites share a number of proteins that are expressed by both stages, including the Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck Proteins (RONs). Although AMA1 and RONs are essential for merozoite invasion of erythrocytes during asexual blood stage replication of the malaria parasite, their precise function in sporozoites is unclear. Here we performed RFP-trap immunoprecipitation experiments using lysates from transgenic sporozoites expressing RON4 fused to mCherry. RON4, RON2, RON5 and AMA1 were the main proteins identified by mass spectrometry among co-precipitated proteins, showing that AMA1-RON interactions are conserved in salivary gland sporozoites.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:57:05.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soumia HAMADA
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-05 14:02:48	ID requested
⏵ 1	2022-08-11 23:57:06	announced

Fernandes P, Loubens M, Le Borgne R, Marinach C, Ardin B, Briquet S, Vincensini L, Hamada S, Hoareau-Coudert B, Verbavatz JM, Weiner A, Silvie O, The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts. PLoS Pathog, 18(6):e1010643(2022) [pubmed]

submitter keyword: Plasmodium, malaria, sporozoites, AMA1, RONs.

Solenne Chardonnet
contact affiliation	Plateforme P3S - UMS PASS 37 - Sorbonne Universite - INSERM
contact email	solenne.chardonnet@sorbonne-universite.fr
lab head
Soumia HAMADA
contact affiliation	Plateforme P3S UMS-37 PASS – Sorbonne Universite
contact email	soumia.hamada@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031463

PRIDE project URI

• PRIDE
  1. PXD031463
     1. Label: PRIDE project
     2. Name: The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts