PXD031437

PXD031437 is an original dataset announced via ProteomeXchange.

Title	Chitinolytic enzymes confer pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis
Description	Aliivibrio (Vibrio) salmonicida is the causative agent of cold-water vibriosis in salmonids (Oncorhynchus mykiss and Salmo salar L.) and gadidae (Gadus morhua L.). Virulence-associated factors that are essential for the full spectrum of Al. salmonicida pathogenicity are largely unknown. Chitin-active lytic polysaccharide monooxygenases (LPMOs) have been indicated to play roles in both chitin degradation and virulence in a variety of pathogenic bacteria. In the present study we investigated the role of LPMOs in the pathogenicity of Al. salmonicida LFI238 in Atlantic salmon (Salmo salar L.). In vivo challenge experiments using isogenic deletion mutants of the two LPMOs encoding genes AsLPMO10A and AsLPMO10B, showed that both LPMOs, and in particular AsLPMO10B, were important in the invasive phase of cold-water vibriosis. Crystallographic analysis of the AsLPMO10B AA10 LPMO domain (to 1.4 Å resolution) revealed high structural similarity to viral fusolin, an LPMO known to enhance the virulence of insecticidal agents. Finally, exposure to Atlantic salmon serum resulted in substantial proteome re-organization of the Al. salmonicida LPMO deletion variants compared to the wild type strain, indicating the struggle of the bacterium to adapt to the host immune components.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:37:27.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Per Kristian Edvardsen
SpeciesList	scientific name: Aliivibrio salmonicida LFI1238; NCBI TaxID: 316275;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-04 15:22:35	ID requested
1	2022-10-13 13:08:41	announced
⏵ 2	2023-11-14 06:37:28	announced	2023-11-14: Updated project metadata.

Sk, å, ne A, Edvardsen PK, Cordara G, Loose JSM, Leitl KD, Krengel U, S, ø, rum H, Askarian F, Vaaje-Kolstad G, Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis. BMC Microbiol, 22(1):194(2022) [pubmed]

submitter keyword: LPMOs,Aliivibrio salmonicida, timsTOF Pro

Gustav Vaaje-Kolstad
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences, 1432, Ås Norway
contact email	gustav.vaaje-kolstad@nmbu.no
lab head
Per Kristian Edvardsen
contact affiliation	Norwegian University of Life Sciences
contact email	per.kristian.edvardsen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031437
     1. Label: PRIDE project
     2. Name: Chitinolytic enzymes confer pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis