PXD031422

PXD031422 is an original dataset announced via ProteomeXchange.

Title	Localisation of the Golgi pH regulator GPR89 extends to the endoplasmic reticulum and confers selective dependency in breast cancer
Description	Identification of targetable biology in breast cancer is an important unmet need, particularly for triple negative breast cancer (TNBC) where outcomes are poor and there are few licensed targeted therapies. Here, we identify the anion channel GPR89 as a novel breast cancer oncogene relevant in TNBC and other subtypes. We demonstrate that GPR89 extends its usual localisation from the Golgi in non-malignant cells to Endoplasmic Reticulum (ER)/Golgi in breast cancer cells where it regulates ER pH. Extended localisation of GPR89, to include mitochondria-ER associated membranes (MAMs), is also associated with GPR89 dependency. We further demonstrate that GPR89 helps breast cancer cells survive ER stress through modulation of the unfolded protein response and helps support cancer cells to rewire their metabolism towards glycolysis by extending localisation to the MAMs. This suggests the GPR89 anion channel as a potential novel drug target relevant to aggressive breast cancers with limited targeted therapy options.
HostingRepository	PRIDE
AnnounceDate	2026-06-02
AnnouncementXML	Submission_2026-06-02_10:34:49.241.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-03 12:34:29	ID requested
⏵ 1	2026-06-02 10:34:49	announced

Dataset with its publication pending

submitter keyword: GPR89,triple negative breast cancer, ER stress, IRE1a

Jyoti Choudhary
contact affiliation	The Institute of Cancer Research
contact email	jyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliation	The Institute of Cancer Research
contact email	james.wright@icr.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031422
     1. Label: PRIDE project
     2. Name: Localisation of the Golgi pH regulator GPR89 extends to the endoplasmic reticulum and confers selective dependency in breast cancer