PXD031420

PXD031420 is an original dataset announced via ProteomeXchange.

Title	Decoding the absolute stoichiometric composition and structural plasticity of α-carboxysomes
Description	Carboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria and some chemoautotrophs. This self-assembling organelle encapsulates the key CO2-fixing enzymes, Rubisco, and carbonic anhydrase using a polyhedral protein shell that is constructed by hundreds of shell protein paralogs. The α-carboxysome from the chemoautotroph Halothiobacillus neapolitanus serves as a model system in fundamental studies and synthetic engineering of carboxysomes. Here we adopt a QconCAT-based quantitative mass spectrometry approach to determine the stoichiometric composition of native α-carboxysomes from H. neapolitanus. We further performed an in-depth comparison of the protein stoichiometry of native and recombinant α-carboxysomes heterologously generated in Escherichia coli to evaluate the structural variability and remodeling of α-carboxysomes. Our results provide insight into the molecular principles that mediate carboxysome assembly, which may aid in rational design and reprogramming of carboxysomes in new contexts for biotechnological applications
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:45:04.012.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031420
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Brownridge
SpeciesList	scientific name: Halothiobacillus neapolitanus c2; NCBI TaxID: 555778;
ModificationList	6x(13)C; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-03 12:25:14	ID requested
⏵ 1	2024-10-07 13:45:04	announced

10.6019/PXD031420;

10.1128/mbio.03629-21;

Sun Y, Harman VM, Johnson JR, Brownridge PJ, Chen T, Dykes GF, Lin Y, Beynon RJ, Liu LN, -Carboxysomes. mBio, 13(2):e0362921(2022) [pubmed]

submitter keyword: carboxysomes

Professor Luning Liu
contact affiliation	Institute of Systems, Molecular and Integrative Biology University of Liverpool Liverpool UK
contact email	lnliu@liverpool.ac.uk
lab head
Philip Brownridge
contact affiliation	University of Liverpool
contact email	philipjb@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031420
     1. Label: PRIDE project
     2. Name: Decoding the absolute stoichiometric composition and structural plasticity of α-carboxysomes