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PXD031420

PXD031420 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDecoding the absolute stoichiometric composition and structural plasticity of α-carboxysomes
DescriptionCarboxysomes are anabolic bacterial microcompartments that play an essential role in carbon fixation in cyanobacteria and some chemoautotrophs. This self-assembling organelle encapsulates the key CO2-fixing enzymes, Rubisco, and carbonic anhydrase using a polyhedral protein shell that is constructed by hundreds of shell protein paralogs. The α-carboxysome from the chemoautotroph Halothiobacillus neapolitanus serves as a model system in fundamental studies and synthetic engineering of carboxysomes. Here we adopt a QconCAT-based quantitative mass spectrometry approach to determine the stoichiometric composition of native α-carboxysomes from H. neapolitanus. We further performed an in-depth comparison of the protein stoichiometry of native and recombinant α-carboxysomes heterologously generated in Escherichia coli to evaluate the structural variability and remodeling of α-carboxysomes. Our results provide insight into the molecular principles that mediate carboxysome assembly, which may aid in rational design and reprogramming of carboxysomes in new contexts for biotechnological applications
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:45:04.012.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031420
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPhilip Brownridge
SpeciesList scientific name: Halothiobacillus neapolitanus c2; NCBI TaxID: 555778;
ModificationList6x(13)C; 6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-03 12:25:14ID requested
12024-10-07 13:45:04announced
Publication List
10.6019/PXD031420;
10.1128/mbio.03629-21;
Sun Y, Harman VM, Johnson JR, Brownridge PJ, Chen T, Dykes GF, Lin Y, Beynon RJ, Liu LN, -Carboxysomes. mBio, 13(2):e0362921(2022) [pubmed]
Keyword List
submitter keyword: carboxysomes
Contact List
Professor Luning Liu
contact affiliationInstitute of Systems, Molecular and Integrative Biology University of Liverpool Liverpool UK
contact emaillnliu@liverpool.ac.uk
lab head
Philip Brownridge
contact affiliationUniversity of Liverpool
contact emailphilipjb@liv.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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