PXD031413

PXD031413 is an original dataset announced via ProteomeXchange.

Title	Label free DIA proteomic analysis of thoracic lymph from cigarette smoke (CS)-exposed mice
Description	The lymphatic vasculature is critical for lung function, but defects in lymphatic functionin the pathogenesis of lung disease is understudied. To further assess the contribution of lymphatics to the pathogenesis of lung emphysema we used a mouse model of cigarette smoke (CS)-induced emphysema, and analyzed lung lymphatics using immunohistochemistry, functional assays, and confocal microscopy. Additionally, we further harvested thoracic lymph from CS-exposed mice for proteomic analysis. In this presented section of our research, we highlight the label free quantitative DIA proteomics approaches used to profile the proteomic and peptidomics changes in the lymph from cigarette smoke (CS)-mice as compared with the lymph proteome from mice exposed to room air. Label free quantitative DIA proteomics analysis of lymph confirmed upregulation of coagulation and inflammatory pathways in the lymphatics of CS-exposed mice compared to control mice.
HostingRepository	PRIDE
AnnounceDate	2022-05-10
AnnouncementXML	Submission_2022-05-10_04:24:43.476.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031413
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cristina Clement
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-03 08:07:57	ID requested
⏵ 1	2022-05-10 04:24:44	announced

Summers BD, Kim K, Clement CC, Khan Z, Thangaswamy S, McCright J, Maisel K, Zamora S, Quintero S, Racanelli AC, Redmond D, D'Armiento J, Yang J, Kuang A, Monticelli L, Kahn ML, Choi AMK, Santambrogio L, Reed HO, Lung lymphatic thrombosis and dysfunction caused by cigarette smoke exposure precedes emphysema in mice. Sci Rep, 12(1):5012(2022) [pubmed]

submitter keyword: label free proteomics

DIA proteomics and peptidomics of mouse lymph

DDA spectral libraries;

Hasina Outtz Reed M.D. PhD
contact affiliation	Division of Pulmonary and Critical Care Medicine Weill Cornell Medicine
contact email	hho2001@med.cornell.edu
lab head
Cristina Clement
contact affiliation	Weill Cornell Medicine
contact email	ccc4002@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD031413

PRIDE project URI

• PRIDE
  1. PXD031413
     1. Label: PRIDE project
     2. Name: Label free DIA proteomic analysis of thoracic lymph from cigarette smoke (CS)-exposed mice