PXD031412

PXD031412 is an original dataset announced via ProteomeXchange.

Title	Proteome dynamics during transition from exponential to stationary phase in yeast
Description	Glycolysis is the central pathway for sugar metabolism in most living organisms. The single celled eukaryote yeast is a widely used model organism for higher eukaryotes in which the regulation of glycolysis is widely studied. S. cerevisiae is one of the few eukaryotic organisms that can efficiently grow under both aerobic and anaerobic conditions. Furthermore, this yeast switches from proliferation into a resting phase when nutrients are exhausted. However, little is known about the proteome dynamics that takes place during this transition, particularly under anaerobic conditions. Moreover, like many other organisms including humans, the genome of S. cerevisiae contains duplications which result in the expression of so called ‘isoenzymes’ in central metabolic pathways including glycolysis. Interestingly, the role of those ‘isoenzymes’ remains elusive to date. Here we describe a large-scale quantitative proteome study combining shotgun experiments using a nano-LC coupled to a QE plus Orbitrap mass spectrometer (Thermo, Germany), to capture the proteome dynamics during transition from proliferation into stationary phase, under both aerobic and anaerobic growth. Furthermore, we explore the proteome dynamics of a mutant yeast where the glycolytic isoenzymes are deleted.
HostingRepository	PRIDE
AnnounceDate	2023-04-15
AnnouncementXML	Submission_2023-04-15_04:14:18.790.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MartinPabst
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; deaminated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-03 08:07:20	ID requested
⏵ 1	2023-04-15 04:14:19	announced

Dataset with its publication pending

submitter keyword: growth,Yeast, TMT quantification, Orbitrap QE plus, glycolysis

MartinPabst
contact affiliation	Biotechnology, Applied Sciences, Delft University of Technology
contact email	m.pabst@tudelft.nl
lab head
MartinPabst
contact affiliation	TUD
contact email	m.pabst@tudelft.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD031412

PRIDE project URI

• PRIDE
  1. PXD031412
     1. Label: PRIDE project
     2. Name: Proteome dynamics during transition from exponential to stationary phase in yeast