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PXD031402

PXD031402 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBiosynthesis of polycyclopropanated high energy biofuels
DescriptionCyclopropanes-functionalized hydrocarbons are excellent fuels. however, their synthesis is challenging and harmful for the environment. In this work we produced polycyclopropanated fatty acids in bacteria. These molecules can be easily converted into renewable fuels for high energy applications such as shipping, long-haul transport, aviation, and rocketry. We explored the chemical diversity encoded in the genome of thousands of bacteria to identify and repurpose naturally occurring cyclopropanated molecules. We identified a set of candidate iterative Polyketide Synthases (iPKSs) predicted to produce polycyclopropanated fatty acids (POP-FAs), expressed these PKSs in Streptomyces coelicolor and produced the POP-FAs. We determined the structure of the molecules and increased their production 22-fold. Polycyclopropanated fatty acid methyl esters (POP-FAMEs) were obtained by methyl esterifying the POP-FAs. Finally, we calculated the enthalpy of combustion of several POP fuel candidates to assess their potential as replacement for fossil fuels in energy demanding applications. Our research shows that POP-FAMEs and other polyketide derived POPs have the energetic properties for energy demanding applications for which sustainable alternatives are scarce.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_06:59:25.285.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD031402
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Petzold
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument6550 iFunnel Q-TOF LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-03 03:36:46ID requested
12022-09-19 11:21:45announced
22023-11-14 06:59:25announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD031402;
Keyword List
submitter keyword: sustainability, polycyclopropanated fatty acid methyl esters, polyketide synthase, POP- FAME,Biofuels, Rocket fuel
Contact List
Chris Petzold
contact affiliationStaff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact emailcjpetzold@lbl.gov
lab head
Christopher Petzold
contact affiliationLawrence Berkeley National Laboratory
contact emailcjpetzold@lbl.gov
dataset submitter
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Dataset FTP location
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