PXD031395

PXD031395 is an original dataset announced via ProteomeXchange.

Title	The AdipoR2 protein recruits an ER-localized fatty acid elongation complex to promote membrane homeostasis
Description	The human AdipoR2 protein and its C. elegans homolog PAQR-2 are multi-pass plasma membrane proteins that protect cells against membrane rigidification. We seek to better understand the mechanisms by which these proteins can promote the synthesis and incorporation of membrane-fluidizing unsaturated fatty acids into phospholipids. To this end, we performed immunoprecipitations of tagged AdipoR2 and PAQR-2 expressed in HEK293 cells or whole C. elegans, respectively, and identified co-immunoprecipitated proteins using mass spectroscopy. Among the conserved interaction partners, we identified many proteins likely important for the protein life cycles (e.g. ribosome, proteasome, vesicle trafficking components). Importantly, several proteins important for fatty acid synthesis, elongation and incorporation into phospholipids were also identified as evolutionarily conserved AdipoR2/PAQR-2 interaction partners. We experimentally verified several of these interactions, and propose that AdipoR2 and PAQR-2 can recruit an ER-bound membrane complex to promote the production and incorporation of unsaturated fatty acids into phospholipids.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:48.002.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Core Facility
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-02 04:22:12	ID requested
1	2023-07-20 10:51:55	announced
⏵ 2	2023-11-14 09:02:56	announced	2023-11-14: Updated project metadata.

Ruiz M, Devkota R, Kaper D, Ruhanen H, Busayavalasa K, Radovi, ć U, Henricsson M, K, ä, kel, ä R, Bor, é, n J, Pilon M, AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidity. J Biol Chem, 299(6):104799(2023) [pubmed]

submitter keyword: PAQR-2, phospholipids, c.elegans,human, AdipoR2

Marc Pilon
contact affiliation	Department of Chemistry and Molecular Biology, University of Gothenburg, Sweden
contact email	marc.pilon@cmb.gu.se
lab head
Proteomics Core Facility
contact affiliation	SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg
contact email	gupcf@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD031395

PRIDE project URI

• PRIDE
  1. PXD031395
     1. Label: PRIDE project
     2. Name: The AdipoR2 protein recruits an ER-localized fatty acid elongation complex to promote membrane homeostasis