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PXD031381

PXD031381 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural requirements for photo-induced RNA-protein cross-linking
DescriptionCross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the UV cross-linking behavior of in vitro reconstituted FOX1 RRM in complex with its cognate RNA sequence, the Fox binding element (FBE), (U)GCAUGU. The FBE heptanucleotide was subsequently mutated, and the impact on UV cross-linking and affinity investigated. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.
HostingRepositoryPRIDE
AnnounceDate2022-06-09
AnnouncementXMLSubmission_2022-06-09_08:34:32.945.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristopher Sarnowski
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-01 03:41:56ID requested
12022-06-09 08:34:33announced
Publication List
Knörlein A, Sarnowski CP, de Vries T, Stoltz M, Götze M, Aebersold R, Allain FH, Leitner A, Hall J, Nucleotide-amino acid π-stacking interactions initiate photo cross-linking in RNA-protein complexes. Nat Commun, 13(1):2719(2022) [pubmed]
Keyword List
submitter keyword: UV cross-linking
MBNL1
FOX1
PTBP1
SF3A1
U1
U2
cross-linking
XL-MS
structural proteomics
protein-RNA
RNA-protein
protein-RNA complex
RNA-protein complex
CLIR-MS
Contact List
Alexander Leitner
contact affiliationETH Zurich, Institute of Molecular Systems Biology
contact emailleitner@imsb.biol.ethz.ch
lab head
Christopher Sarnowski
contact affiliationInstitute of Molecular Systems Biology, Department of Biology, ETH Zürich
contact emailsarnowski@imsb.biol.ethz.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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